Rabbit anti-Human PIK3CA Polyclonal Antibody | anti-PIK3CA antibody

PIK3CA (Y317) (Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit alpha Isoform, PtdIns-3-kinase Subunit alpha, PI3-kinase Subunit alpha, PI3K-alpha, Phosphatidylinositol-4,5-bisphosphate 3-kinase 110kD Catalytic Subunit alpha, PtdIns-3-kina

Cat. Num. AAA628347

• Gene Names: PIK3CA; PI3K; MGC142161; MGC142163; p110-alpha
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PIK3CA; Polyclonal Antibody; PIK3CA (Y317) (Phosphatidylinositol-4; 5-bisphosphate 3-kinase Catalytic Subunit alpha Isoform; PtdIns-3-kinase Subunit alpha; PI3-kinase Subunit alpha; PI3K-alpha; Phosphatidylinositol-4; 5-bisphosphate 3-kinase 110kD Catalytic Subunit alpha; PtdIns-3-kina; Anti -PIK3CA (Y317) (Phosphatidylinositol-4; anti-PIK3CA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PK3CA.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PIK3CA antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding Y317 of human PK3CA (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse heart tissue lysates (35ug/lane) using MBS628347. PK3CA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MBS628347, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using MBS628347 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PIK3CA antibody

Phosphatidylinositol 3-kinase is composed of an 85kD regulatory subunit and a 110kD catalytic subunit. This protein represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2.

Product Categories/Family for anti-PIK3CA antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 54792082
• NCBI GeneID: 5290
• NCBI Accession #: NP_006209.2
• NCBI GenBank Nucleotide #: NM_006218.2
• UniProt Accession #: P42336; Q14CW1; Q99762; Q06DD2; Q0PN16; Q4LE51
• Molecular Weight: 124,284 Da
• NCBI Official Full Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• NCBI Official Symbol: PIK3CA
• NCBI Official Synonym Symbols: PI3K; MGC142161; MGC142163; p110-alpha
• NCBI Protein Information: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3K-alpha; OTTHUMP00000211945; PtdIns-3-kinase p110; PI3-kinase subunit alpha; PI3-kinase p110 subunit alpha; ptdIns-3-kinase subunit alpha; ptdIns-3-kinase subunit p110-alpha; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
• UniProt Protein Name: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• Protein Family: Phosphatidylinositol 4,5-bisphosphate 3-kinase
• UniProt Gene Name: PIK3CA
• UniProt Entry Name: PK3CA_HUMAN

NCBI Description

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq]

Uniprot Description

PIK3CA: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS1 and KRAS. Interaction with HRAS1/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2. Belongs to the PI3/PI4-kinase family.

Protein type: Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate; Oncoprotein; EC 2.7.1.153; Kinase, lipid; EC 2.7.11.1

Chromosomal Location of Human Ortholog: 3q26.3

Cellular Component: lamellipodium; plasma membrane; cytosol; phosphoinositide 3-kinase complex

Molecular Function: protein serine/threonine kinase activity; protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; 1-phosphatidylinositol-3-kinase activity; insulin receptor substrate binding; kinase activity; protein kinase activator activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; protein amino acid phosphorylation; T cell receptor signaling pathway; vasculature development; phosphoinositide phosphorylation; phospholipid metabolic process; protein kinase B signaling cascade; angiogenesis; negative regulation of neuron apoptosis; cardiac muscle contraction; epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; glucose metabolic process; regulation of multicellular organism growth; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase activity; T cell costimulation; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; endothelial cell migration; innate immune response; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Cowden Syndrome 5; Gastric Cancer; Keratosis, Seborrheic; Breast Cancer; Lung Cancer; Ovarian Cancer; Colorectal Cancer; Congenital Lipomatous Overgrowth, Vascular Malformations, And Epidermal Nevi; Megalencephaly-capillary Malformation-polymicrogyria Syndrome; Hepatocellular Carcinoma; Nevus, Epidermal