Mouse anti-Rat TUFM Polyclonal Antibody | anti-Tufm antibody

TUFM (Elongation Factor Tu, Mitochondrial, EF-Tu, P43)

Cat. Num. AAA628068

• Gene Names: Tufm; EFTU; C76308; C76389; EF-TuMT; 2300002G02Rik
• Reactivity: Rat
• Applications: Immunofluorescence, Western Blot
• Purity: Purified
• Synonyms: TUFM; Polyclonal Antibody; TUFM (Elongation Factor Tu; Mitochondrial; EF-Tu; P43); Anti -TUFM (Elongation Factor Tu; anti-Tufm antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TUFM.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.4
• Concentration: 0.5 mg/mL (varies by lot)
• Sequence: MTTMAAATLLRATPHFSGLAAGRTFLLQGLLRLLKAPALPLLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

• Applicable Applications for anti-Tufm antibody: Suitable for use in Western Blot and Immunofluorescence.; ; Other applications not tested.
• Application Notes: Immunofluorescence: 10 ug/mL; ; Optimal dilutions to be determined by the researcher.
• Immunogen: Full-length protein corresponding to aa1-455 from human TUFM.
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of TUFM expression in rat brain using MBS628068.)

Western Blot (WB)

(Western Blot analysis of TUFM expression in HeLa using MBS628068.)

Western Blot (WB)

(Western Blot analysis of TUFM expression in transfected 293T cell line by MBS628068. Lane 1: TUFM transfected lysate (50.05kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of MBS628068 (10ug/ml) on HeLa cell.)

Related Product Information for anti-Tufm antibody

TUFM is a protein which participates in protein translation in mitochondria. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Product Categories/Family for anti-Tufm antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 38173913
• NCBI GeneID: 233870
• UniProt Accession #: Q8BFR5; Q497E7; Q6P919
• NCBI Official Full Name: Tufm protein
• NCBI Official Synonym Full Names: Tu translation elongation factor, mitochondrial
• NCBI Official Symbol: Tufm
• NCBI Official Synonym Symbols: EFTU; C76308; C76389; EF-TuMT; 2300002G02Rik
• NCBI Protein Information: elongation factor Tu, mitochondrial
• UniProt Protein Name: Elongation factor Tu, mitochondrial
• UniProt Gene Name: Tufm
• UniProt Entry Name: EFTU_MOUSE

Uniprot Description

EFTU: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Defects in TUFM are the cause of combined oxidative phosphorylation deficiency type 4 (COXPD4). COXPD4 is characterized by neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Protein type: Mitochondrial; Translation elongation; Translation; RNA-binding

Cellular Component: membrane; mitochondrion; mitochondrial inner membrane; intracellular; myelin sheath

Molecular Function: GTPase activity; GTP binding; nucleotide binding; translation elongation factor activity

Biological Process: translational elongation; translation