Rabbit p27Kip1, Polyclonal Antibody

p27Kip1, phosphorylated, (Ser10)

Cat. Num. AAA627899

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: p27Kip1; ; Polyclonal Antibody; phosphorylated; (Ser10); Anti -p27Kip1; anti-p27Kip1; antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes p27 when it is phosphorylated at the Ser10 residue. Lysates of HEK 293T cells transfected with an expression plasmid encoding a p27 mutant where Ser10 was substituted with an alanine residue were used to confirm the antibody's specificity. Reactivity was confirmed with HEK293 cells expressing wild-type p27/KIP that is normally phosphorylated. NIH3T3 and MCF-7 cell lysates can also be used as positive controls.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

• Applicable Applications for anti-p27Kip1, antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunopreciptiation and Immunofluorescence.; Dilution: Western Blot: 1-3ug/ml; Immunoprecipitation: 5-10ug/IP reaction; Immunofluorescence: 5-10ug/ml; Optimal dilutions to be determined by the researcher
• Immunogen: Synthetic peptide encompassing the phosphorylated Ser10 residue of p27/Kip1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-p27Kip1, antibody

Cyclin dependent kinases (Cdk) govern cell cycle progression in eukaryotic cells. Various cyclin dependent kinase inhibitors may suppress the activity of these proteins. There are two families of Cdk inhibitors, the cip/kip (kinase inhibitor protein) and ink (inhibitor of CDK4) families. The cip/kip inhibitors share sequence homology and can inhibit the kinase activities of a variety of Cdks. Cdk inhibitor p27(Kip1) is a member of the kip family and negatively regulates G1 progression by binding to cyclin-Cdk2 complexes. The concentration and subcellular localization of p27 controls its activity. Cellular abundance of p27 is regulated by its degradation via the ubiquitin-proteasome pathway. p27 needs to be transported into the nucleus to exert its inhibitory action. Nuclear import is dependent on the presence of a nuclear localization signal near the C-terminus of the protein, whereas nuclear export into the cytoplasm is dependent on Ser10 phosphorylation. Export into the cytoplasm has been suggested as a mechanism to remove a fraction of p27 from its nuclear targets thereby lowering its effective nuclear concentration below a critical threshold resulting in activation of cyclin-Cdk2 complexes. There have been several reports that indicate p27 (Kip1) mediates multiple cellular functions such as cell proliferation, cell differentiation, and apoptosis. The pivotal role of p27 in these cellular functions makes it important in many disease processes, including neoplasia, and its reduced expression has been shown to correlate with poor prognosis in cancer patients.

Product Categories/Family for anti-p27Kip1, antibody

Antibodies; Abs to Protein Kinase Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 4587316
• NCBI Official Full Name: p27kip1

NCBI Description

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]

Uniprot Description

p27Kip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. May mediate TGF beta-induced g1 arrest. Its degradation is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Protein type: Oncoprotein; Inhibitor; Cell cycle regulation; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 12p13.1-p12

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol; endosome

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; chaperone binding; protein complex binding; Hsp70 protein binding; caspase activator activity; protein phosphatase binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: negative regulation of kinase activity; response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; positive regulation of microtubule polymerization; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to estradiol stimulus; negative regulation of cell proliferation; sensory perception of sound; positive regulation of cell proliferation; response to glucose stimulus; negative regulation of mitotic cell cycle; cell cycle arrest; inner ear development; potassium ion transport; response to drug; caspase activation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of cyclin-dependent protein kinase activity; negative regulation of cell motility; response to amino acid stimulus; response to cadmium ion; autophagic cell death; response to hypoxia; positive regulation of protein catabolic process; innate immune response; negative regulation of phosphorylation; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; G1/S transition of mitotic cell cycle

Disease: Multiple Endocrine Neoplasia, Type Iv