Rabbit anti-Human GNL3 Polyclonal Antibody | anti-GNL3 antibody

GNL3 Antibody

Cat. Num. AAA9400692

• Gene Names: GNL3; NS; E2IG3; NNP47; C77032
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: GNL3; Polyclonal Antibody; GNL3 Antibody; NS; E2IG3; NNP47; C77032; anti-GNL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total GNL3 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 2.8 mg/ml (varies by lot)
• Sequence Length: 549

• Applicable Applications for anti-GNL3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:2000; Immunohistochemistry: 1:50-1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Fusion protein corresponding to a region derived from internal residues of human guanine nucleotide binding protein-like 3
• Target Name: GNL3
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 6%SDS-PAGE Lysates (from left to right): K562, Hela, LoVo, 293T and Raji cell Amount of lysate: 40ug per lane Primary antibody: 1/300 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/60.)

Related Product Information for anti-GNL3 antibody

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.

Product Categories/Family for anti-GNL3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 45593130
• NCBI GeneID: 26354
• NCBI Accession #: NP_055181.3
• NCBI GenBank Nucleotide #: NM_014366.4
• UniProt Accession #: Q9BVP2; Q5PU80; Q96SV6; Q96SV7; Q9UJY0; B2RDC1
• Molecular Weight: 60,540 Da
• NCBI Official Full Name: guanine nucleotide-binding protein-like 3 isoform 1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein-like 3 (nucleolar)
• NCBI Official Symbol: GNL3
• NCBI Official Synonym Symbols: NS; E2IG3; NNP47; C77032
• NCBI Protein Information: guanine nucleotide-binding protein-like 3
• UniProt Protein Name: Guanine nucleotide-binding protein-like 3
• Protein Family: Guanine nucleotide-binding protein
• UniProt Gene Name: GNL3
• UniProt Synonym Gene Names: E2IG3; NS; NNP47
• UniProt Entry Name: GNL3_HUMAN

NCBI Description

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

GNL3: May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis. May interact with p53/TP53 via its basic domain. Increased levels in lung tissue in cancer patients. Belongs to the MMR1/HSR1 GTP-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Cell cycle regulation; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: nucleoplasm; extracellular space; membrane; nucleolus; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: cell proliferation; metabolic process; ribosome biogenesis and assembly; regulation of cell proliferation