Rabbit anti-Human BCL2L12 Polyclonal Antibody | anti-BCL2L12 antibody

BCL2L12, NT (BCL2L12, BPR, Bcl-2-like protein 12, Bcl-2-related proline-rich protein) (Azide free) (HRP)

Cat. Num. AAA6277981

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BCL2L12; Polyclonal Antibody; NT (BCL2L12; BPR; Bcl-2-like protein 12; Bcl-2-related proline-rich protein) (Azide free) (HRP); Bcl-2-related proline-rich protein; anti-BCL2L12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BCL2L12 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BCL2L12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-45 amino acids from the N-terminal region of human BCL2L12.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BCL2L12 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the BCL2L12 antibody detected the BCL2L12 protein (arrow).)

Related Product Information for anti-BCL2L12 antibody

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero-or homodimers and act as anti-or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 2 (BH2). The function of this gene has not yet been determined. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq].

Product Categories/Family for anti-BCL2L12 antibody

Antibodies; Apoptosis

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Florou, D., et al. Biochem. Biophys. Res. Commun. 391(1):214-218(2010). Hong, Y., et al. Mol. Cell. Biochem. 333 (1-2), 323-330 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Thomadaki, H., et al. Ann. N. Y. Acad. Sci. 1171, 276-283 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 100913021
• NCBI GeneID: 83596
• NCBI Accession #: NP_001035758.1
• NCBI GenBank Nucleotide #: NM_001040668.1
• UniProt Accession #: Q9HB09; Q3SY11; Q3SY13; Q96I96; Q9HB08
• Molecular Weight: 36,821 Da
• NCBI Official Full Name: bcl-2-like protein 12 isoform 3
• NCBI Official Synonym Full Names: BCL2-like 12 (proline rich)
• NCBI Official Symbol: BCL2L12
• NCBI Protein Information: bcl-2-like protein 12; bcl2-L-12; Bcl-2 related proline-rich protein; bcl-2-related proline-rich protein
• UniProt Protein Name: Bcl-2-like protein 12
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L12
• UniProt Synonym Gene Names: BPR; Bcl2-L-12
• UniProt Entry Name: B2L12_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 2 (BH2). The function of this gene has not yet been determined. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Bcl-2L12: belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 2 (BH2). The function of this gene has not yet been determined. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: membrane; nucleus

Molecular Function: protein binding; p53 binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter