mouse anti-Human Homeobox Protein DLX-5 Monoclonal Antibody | anti-DLX5 antibody

Homeobox Protein DLX-5 (DLX5)

Cat. Num. AAA606351

• Gene Names: DLX5; DLX5
• Reactivity: Human
• Applications: ELISA
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Homeobox Protein DLX-5; Monoclonal Antibody; Homeobox Protein DLX-5 (DLX5); Anti -Homeobox Protein DLX-5 (DLX5); anti-DLX5 antibody

• Host: mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9G371
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.

• Applicable Applications for anti-DLX5 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.
• Immunogen: Partial recombinant protein of human homeobox protein DLX-5 (aa1-10) with a GST tag.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EIA)

(Sandwhich ELISA using MBS606351, as a capture antibody. The detection limit for recombinant GST tagged homeobox protein DLX-5 is 0.3ng/ml.)

Related Product Information for anti-DLX5 antibody

This protein belongs to the the distal-less homeobox family. It contains one homeobox DNA-binding domain. It is thought to play a role in bone development and fracture healing. It is first expressed at 8.5 to 9 days in facial and branchial arch mesenchyme, otic vesicles and frontonasal ectoderm around olfactory placodes. At day 9.5-10, expression is seen in the developing forebrain. In day 12.5 embryos it is expressed in the brain and bones and also in all skeletal structures of midgestattion embryos after the first catilage formation. Expression remains unaltered in both brain and skeleton in day 15 embryos and slowly decreases in day 17 embryos. The unprocessed precursor has a length of 289 amino acids and a predicted molecular weight of 31.5kD.

Product Categories/Family for anti-DLX5 antibody

Antibodies; Abs to Homeobox (HOX)

NCBI and Uniprot Product Information

• NCBI GI #: 4885187
• NCBI GeneID: 1749
• NCBI Accession #: NP_005212.1
• NCBI GenBank Nucleotide #: NM_005221.5
• UniProt Accession #: P56178; Q9UPL1; Q53Y73
• Molecular Weight: 31,540 Da
• NCBI Official Full Name: homeobox protein DLX-5
• NCBI Official Synonym Full Names: distal-less homeobox 5
• NCBI Official Symbol: DLX5
• NCBI Official Synonym Symbols: DLX5
• NCBI Protein Information: homeobox protein DLX-5; OTTHUMP00000205292; distal-less homeo box 5
• UniProt Protein Name: Homeobox protein DLX-5
• Protein Family: Homeobox protein
• UniProt Gene Name: DLX5
• UniProt Entry Name: DLX5_HUMAN

NCBI Description

This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein may play a role in bone development and fracture healing. Mutation in this gene, which is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 7, may be associated with split-hand/split-foot malformation. [provided by RefSeq]

Uniprot Description

DLX5: Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding. Defects in DLX5 are the cause of split-hand/foot malformation type 1, with sensorineural hearing loss (SHFM1D). A disease characterized by the association of split- hand/foot malformation with deafness. Split-hand/foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. Some patients have been found to have mental retardation, ectodermal and craniofacial findings, and orofacial clefting. Belongs to the distal-less homeobox family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: nuclear chromatin; cytoplasm

Biological Process: nervous system development; axon guidance; inner ear morphogenesis; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; olfactory pit development; palate development; BMP signaling pathway; osteoblast differentiation; cell proliferation; positive regulation of osteoblast differentiation; epithelial cell differentiation; anatomical structure formation; skeletal development; positive regulation of epithelial cell proliferation; endochondral ossification; embryonic limb morphogenesis

Disease: Split-hand/foot Malformation 1 With Sensorineural Hearing Loss, Autosomal Recessive