Rabbit anti-Human, Mouse AKTIP Polyclonal Antibody | anti-AKTIP antibody

AKTIP, CT (AKTIP, FTS, AKT-interacting protein, Ft1, Fused toes protein homolog) (APC)

Cat. Num. AAA6272697

• Gene Names: AKTIP; FT1; FTS
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AKTIP; Polyclonal Antibody; CT (AKTIP; FTS; AKT-interacting protein; Ft1; Fused toes protein homolog) (APC); Fused toes protein homolog; anti-AKTIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-AKTIP antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AKTIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-288 amino acids from the C-terminal region of human AKTIP.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(AKTIP Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the AKTIP antibody detected the AKTIP protein (arrow).)

Related Product Information for anti-AKTIP antibody

The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein.

Product Categories/Family for anti-AKTIP antibody

Antibodies; Proteins

References

Notaridou, M., et al. Int. J. Cancer (2010). In press: Quaye, L., et al. Hum. Mol. Genet. 18(10):1869-1878(2009). Xu, L., et al. Mol. Biol. Cell 19(12):5059-5071(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 61743933
• NCBI GeneID: 64400
• NCBI Accession #: NP_001012398.1
• NCBI GenBank Nucleotide #: NM_001012398.1
• UniProt Accession #: Q9H8T0; Q503B1; Q53H38
• Molecular Weight: 33,128 Da
• NCBI Official Full Name: AKT-interacting protein
• NCBI Official Synonym Full Names: AKT interacting protein
• NCBI Official Symbol: AKTIP
• NCBI Official Synonym Symbols: FT1; FTS
• NCBI Protein Information: AKT-interacting protein; fused toes homolog; fused toes protein homolog
• UniProt Protein Name: AKT-interacting protein
• Protein Family: AKT-interacting protein
• UniProt Gene Name: AKTIP
• UniProt Synonym Gene Names: FTS
• UniProt Entry Name: AKTIP_HUMAN

NCBI Description

The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade. Ref.6 Ref.7

Subunit structure: Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with HOOK1, HOOK2 and HOOK3. The FHF complex associates with the homotypic vesicular sorting complex (the HOPS complex). Also interacts with AKT1. Ref.6 Ref.7

Subcellular location: Cytoplasm. Cell membrane; Peripheral membrane protein Ref.6.

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family. FTS subfamily.

Caution: Lacks the conserved Cys residue necessary for ubiquitin-conjugating enzyme E2 activity.