Human Interleukin 18 ELISA Kit | IL-18 elisa kit

Human Interleukin 18 ELISA Kit

Cat. Num. AAA760823

• Gene Names: IL18; IGIF; IL-18; IL-1g; IL1F4
• Reactivity: Human
• Synonyms: Interleukin 18; Human Interleukin 18 ELISA Kit; IL-18 (Interleukin 18)/IL18/IGIF/IL-1g/IL-1F4/Interferon-gamma-inducing factor; IL-18 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL-18. No significant cross-reactivity or interference between IL-18 and analogues was observed.
• Sequence Length: 193

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-18 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504653
• NCBI GeneID: 3606
• NCBI Accession #: NP_001553.1
• NCBI GenBank Nucleotide #: NM_001562.3
• Molecular Weight: 21,896 Da
• NCBI Official Full Name: interleukin-18 isoform 1
• NCBI Official Synonym Full Names: interleukin 18
• NCBI Official Symbol: IL18
• NCBI Official Synonym Symbols: IGIF; IL-18; IL-1g; IL1F4
• NCBI Protein Information: interleukin-18
• UniProt Protein Name: Interleukin-18
• UniProt Gene Name: IL18
• UniProt Synonym Gene Names: IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma
• UniProt Entry Name: IL18_HUMAN

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: cytokine activity; protein binding

Biological Process: angiogenesis; cell-cell signaling; chemokine biosynthetic process; granulocyte macrophage colony-stimulating factor biosynthetic process; immune response; inflammatory response; interferon-gamma biosynthetic process; interleukin-13 biosynthetic process; interleukin-2 biosynthetic process; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; natural killer cell activation; negative regulation of myoblast differentiation; positive regulation of activated T cell proliferation; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; positive regulation of NF-kappaB import into nucleus; positive regulation of NK T cell proliferation; positive regulation of tissue remodeling; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of cell adhesion; sleep; T-helper 1 type immune response; T-helper 2 type immune response