Immunohistochemistry (IHC)
(Immunohistochemistry of formalin-fixed, paraffin-embedded human leiomyoma using MBS633240 followed by peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.)
Mouse Actin, alpha, Smooth Muscle Monoclonal Antibody
Actin, alpha, Smooth Muscle (BSA, Glycerol & Azide Free) (HRP)
Reactivity
Bovine, Chicken, Human, Mouse, Rabbit, Rat
Applications
Immunofluorescence, Immunohistochemistry
Purity
Purified by Protein A Affinity Chromatography
Synonyms
Actin; alpha; Smooth Muscle; Monoclonal Antibody; Smooth Muscle (BSA; Glycerol & Azide Free) (HRP); anti-Actin; Smooth Muscle antibody
Host
Mouse
Reactivity
Bovine, Chicken, Human, Mouse, Rabbit, Rat
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
0.N.5
Specificity
Recognizes the alpha-smooth muscle isoform of actin. Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. Reacts with tumors arising from smooth muscles and myoepithelial cells. Does not crossreact with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial). Species Crossreactivity: Human, baboon, bovine, rabbit, mouse, rat and chicken.
Purity/Purification
Purified by Protein A Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-Actin, alpha, Smooth Muscle antibody
Immunofluorescence (IF), Immunohistochemistry (IHC)
Immunogen
N-terminal decapeptide of the alpha smooth muscle isoform of actin; acetylated at the N-terminus. Epitope Location: Acetyl group and the first 4 amino acids on the N-terminal end of the peptidic chain of alpha-smooth actin. Cellular Localization: Cytoplasmic.
Conjugate
HRP
Preparation and Storage
Store at -20 degree C.
Immunohistochemistry (IHC)
(Immunohistochemistry of formalin-fixed, paraffin-embedded human leiomyoma using MBS633240 followed by peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.)
Immunohistochemistry (IHC)
(Immunohistochemistry of formalin-fixed, paraffin-embedded human leiomyoma using MBS633240 followed by peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.)
Product Categories/Family for anti-Actin, alpha, Smooth Muscle antibody
References
1. Bochaton-Piallat ML; Gabbiani F; Gabbiani G. Heterogeneity of rat aortic smooth muscle cell replication during development: correlation with replicative activity after experimental endothelial denudation in adults. Journal of Submicroscopic Cytology and Pathology, 1994, 26(1):1-8. 2. Cremona O; Savoia P; Marchisio PC; Gabbiani G; Chaponnier C. The alpha 6 and beta 4 integrin subunits are expressed by smooth muscle cells of human small vessels: a new localization in mesenchymal cells. Journal of Histochemistry and Cytochemistry, 1994, 42(9):1221-8. 3. Estes JM; Vande Berg JS; Adzick NS; MacGillivray TE; Desmouliere A; Gabbiani G. Phenotypic and functional features of myofibroblasts in sheep fetal wounds. Differentiation, 1994, 56(3):173-81. 4. Orlandi A; Ehrlich HP; Ropraz P; Spagnoli LG; Gabbiani G. Rat aortic smooth muscle cells isolated from different layers and at different times after endothelial denudation show distinct biological features in vitro. Arteriosclerosis and Thrombosis, 1994, 14(6):982-9.
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Product Notes
The Actin, alpha, Smooth Muscle (Catalog #AAA6266634) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Actin, alpha, Smooth Muscle (BSA, Glycerol & Azide Free) (HRP) reacts with Bovine, Chicken, Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Actin, alpha, Smooth Muscle can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the Actin, alpha, Smooth Muscle for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, alpha, Smooth Muscle, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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