Rabbit anti-Human Heat Shock Factor 1 Polyclonal Antibody

Heat Shock Factor 1, phosphorylated (Ser307) (HSF1, Heat Shock Factor Protein 1, HSF 1, Heat Shock Transcription Factor 1, HSTF 1, HSTF1)

Cat. Num. AAA626250

• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Heat Shock Factor 1; Polyclonal Antibody; phosphorylated (Ser307) (HSF1; Heat Shock Factor Protein 1; HSF 1; Heat Shock Transcription Factor 1; HSTF 1; HSTF1); Anti -Heat Shock Factor 1; anti-Heat Shock Factor 1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HSF1 when phosphorylated at serine 307.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Heat Shock Factor 1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 1:100-1:200; Western Blot: 1:500-1:1,000
• Immunogen: Synthetic phosphopeptide corresponding to human HSF1 around the phosphorylation site of serine 307 (P-Q-SP-P-R).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Heat Shock Factor 1 antibody

HSF1 belongs to a family of Heat Shock transcription Factors (HSFs) that bind to highly conserved heat shock elements (HSEs) in the promoter regions of heat shock genes and regulate the expression of heat shock proteins (Hsps). Multiple HSFs exist in higher eukaryotes whereas yeast and Drosophila have only a single HSF. HSF1, HSF2, HSF3 have been reported in chicken, HSF1 and HSF2 in mouse and HSF1, HSF2, and HSF4 in human. Most HSFs have several common functional motifs whose amino acid sequences are highly conserved. At the N-terminus of all HSFs there is a DNA binding domain essential for binding to the HSE. Hydrophobic repeats located adjacent to the DNA binding domain are essential for the formation of active trimers which enable all HSFs to specifically bind to DNA. Another short hydrophobic repeat located towards the C-terminal region of most HSFs is thought to be necessary for suppression of trimerization except in the case of yeast HSF and human HSF4. The mouse HSF1 exists as two isoforms which arise from alternative splicing of the HSF1 pre-mRNA. In higher eukaryotes, HSF1 is predominantly found in a diffuse cytoplasmic and nuclear distribution in unstressed cells. On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution. Similar to HSF1, murine HSF2 also exist as two isoforms, the smaller HSF2-beta isoform and the longer HSF2-alpha which has been shown to be more transcriptionally active. Constitutive binding of HSF2 to HSE has been found with spermatogenic cells post-implantation embryos and embryonal carcinoma cells in mouse and these observations suggest that HFS2 may have roles in differentiation and development.

Product Categories/Family for anti-Heat Shock Factor 1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 184403
• NCBI Official Full Name: heat shock factor 1

NCBI Description

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; protein complex; pronucleus; cytoplasm; cytosol

Molecular Function: protein binding; chromatin binding; transcription factor activity

Biological Process: embryonic placenta development; embryonic process involved in female pregnancy; female meiosis; defense response; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; positive regulation of multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; negative regulation of cell proliferation; mRNA transcription; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis