Rabbit anti-Human, Mouse HSF1 Polyclonal Antibody | anti-HSF1 antibody

HSF1 (Phospho-Thr142) Antibody

Cat. Num. AAA9411973

• Gene Names: HSF1; HSTF1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: HSF1; Polyclonal Antibody; HSF1 (Phospho-Thr142) Antibody; HSF 1; HSTF1; Heat shock factor protein 1; anti-HSF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of HSF1 only when phosphorylated at threonine 142.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 529

• Applicable Applications for anti-HSF1 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of threonine 142(L-L-T(p)-D-V) derived from Human HSF1.
• Modification: Phospho-Thr142
• Target Name: HSF1
• Preparation and Storage: Store at -20 degree C/1 year

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human brain tissue using HSF1 (Phospho-Thr142) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Related Product Information for anti-HSF1 antibody

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Rabindran S.K., Proc. Natl. Acad. Sci. U.S.A. 88:6906-6910(1991).The MGC Project Team; Genome Res. 14:2121-2127(2004).Schuetz T.J.,Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915(1991)

Product Categories/Family for anti-HSF1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5031767
• NCBI GeneID: 3297
• NCBI Accession #: NP_005517.1
• NCBI GenBank Nucleotide #: NM_005526.3
• UniProt Accession #: Q00613; Q53XT4; A8K4L0; A8MW26
• Molecular Weight: 52,881 Da
• NCBI Official Full Name: heat shock factor protein 1
• NCBI Official Synonym Full Names: heat shock transcription factor 1
• NCBI Official Symbol: HSF1
• NCBI Official Synonym Symbols: HSTF1
• NCBI Protein Information: heat shock factor protein 1
• UniProt Protein Name: Heat shock factor protein 1
• Protein Family: Heat shock factor protein
• UniProt Gene Name: HSF1
• UniProt Synonym Gene Names: HSTF1; HSF 1; HSTF 1
• UniProt Entry Name: HSF1_HUMAN

NCBI Description

The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016]

Uniprot Description

HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; protein complex; pronucleus; cytoplasm; cytosol

Molecular Function: protein binding; chromatin binding; transcription factor activity

Biological Process: negative regulation of cell proliferation; embryonic placenta development; embryonic process involved in female pregnancy; mRNA transcription; female meiosis; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; response to lipopolysaccharide; defense response; negative regulation of tumor necrosis factor production; positive regulation of multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation