Rabbit LONP2 Polyclonal Antibody | anti-LONP2 antibody

LONP2 Polyclonal Antibody

Cat. Num. AAA3007680

• Gene Names: LONP2; LONP; PLON; LONPL; PSLON
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: LONP2; Polyclonal Antibody; LONP2 Polyclonal Antibody; Lon protease homolog 2; peroxisomal; Lon protease-like protein 2; Lon protease 2; Peroxisomal Lon protease; LONP; anti-LONP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LONP2 pAb detects endogenous levels of LONP2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 852

• Applicable Applications for anti-LONP2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 780-830 of Human LONP2
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of LONP2 pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:H9C2 whole cell lysate (40ug))

Related Product Information for anti-LONP2 antibody

LONP2 (lon peptidase 2, peroxisomal), also known as LONP, LONPL or peroxisomal lon protease, is an 852 amino acid protein belonging to the peptidase S16 family. LONP2 assists in import of peroxisomal matrix proteins and is required for PTS2 (type 2 peroxisome targeting signal)-containing protein processing. As an ATP-dependent serine protease, LONP2 plays a role in degradation of polypeptides that are unassembled or incorrectly folded. The gene encoding LONP2 maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohns disease, which is a gastrointestinal inflammatory condition.

NCBI and Uniprot Product Information

• NCBI GI #: 31377667
• NCBI GeneID: 83752
• NCBI Accession #: NP_113678.2
• NCBI GenBank Nucleotide #: NP_113678.2
• UniProt Accession #: Q86WA8
• Molecular Weight: ~ 96kDa
• NCBI Official Full Name: lon protease homolog 2, peroxisomal isoform 1
• NCBI Official Synonym Full Names: lon peptidase 2, peroxisomal
• NCBI Official Symbol: LONP2
• NCBI Official Synonym Symbols: LONP; PLON; LONPL; PSLON
• NCBI Protein Information: lon protease homolog 2, peroxisomal
• UniProt Protein Name: Lon protease homolog 2, peroxisomal
• UniProt Gene Name: LONP2
• UniProt Synonym Gene Names: LONP; Lon protease 2
• UniProt Entry Name: LONP2_HUMAN

NCBI Description

In human, peroxisomes function primarily to catalyze fatty acid beta-oxidation and, as a by-product, produce hydrogen peroxide and superoxide. The protein encoded by this gene is an ATP-dependent protease that likely plays a role in maintaining overall peroxisome homeostasis as well as proteolytically degrading peroxisomal proteins damaged by oxidation. The protein has an N-terminal Lon N substrate recognition domain, an ATPase domain, a proteolytic domain, and, in some isoforms, a C-terminal peroxisome targeting sequence. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2017]

Uniprot Description

LONP2: ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import. May indirectly regulate peroxisomal fatty acid beta-oxidation through degradation of the self-processed forms of TYSND1. Belongs to the peptidase S16 family.

Protein type: Protease; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: peroxisomal matrix; membrane; peroxisome; nucleus

Molecular Function: peptidase activity; protein binding; enzyme binding; protease binding; ATP-dependent peptidase activity; serine-type endopeptidase activity; ATP binding; receptor binding

Biological Process: protein targeting to peroxisome; peroxisome organization and biogenesis; misfolded or incompletely synthesized protein catabolic process; protein import into peroxisome matrix; regulation of fatty acid beta-oxidation; protein processing; response to organic cyclic substance