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Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with STX3 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Rabbit anti-Human STX3 Polyclonal Antibody | anti-STX3 antibody

STX3, ID (Syntaxin-3, STX3A)

Gene Names
STX3; STX3A; FLJ30906
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified
Purified by ammonium sulfate precipitation.
Synonyms
STX3; Polyclonal Antibody; ID (Syntaxin-3; STX3A); Anti -STX3; anti-STX3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human STX3.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-STX3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Immunogen
Synthetic peptide selected from the Center region of human STX3 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with STX3 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with STX3 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of anti-STX3 Antibody (Center) in A375 cell line lysates (35ug/lane). STX3(arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-STX3 Antibody (Center) in A375 cell line lysates (35ug/lane). STX3(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of STX3 (arrow) using rabbit polyclonal STX3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STX3 gene.)

Western Blot (WB) (Western blot analysis of STX3 (arrow) using rabbit polyclonal STX3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STX3 gene.)
Related Product Information for anti-STX3 antibody
STX3 is otentially involved in docking of synaptic vesicles at presynaptic active zones.
Product Categories/Family for anti-STX3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,155 Da
NCBI Official Full Name
syntaxin-3 isoform 2
NCBI Official Synonym Full Names
syntaxin 3
NCBI Official Symbol
STX3
NCBI Official Synonym Symbols
STX3A; FLJ30906
NCBI Protein Information
syntaxin-3; syntaxin 3A; OTTHUMP00000235765; OTTHUMP00000235767; OTTHUMP00000235768
UniProt Protein Name
Syntaxin-3
Protein Family
UniProt Gene Name
STX3
UniProt Synonym Gene Names
STX3A
UniProt Entry Name
STX3_HUMAN

NCBI Description

The gene is a member of the syntaxin family. The encoded protein is targeted to the apical membrane of epithelial cells where it forms clusters and is important in establishing and maintaining polarity necessary for protein trafficking involving vesicle fusion and exocytosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

STX3: Potentially involved in docking of synaptic vesicles at presynaptic active zones. Belongs to the syntaxin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: SNARE complex; specific granule; azurophil granule; synaptic vesicle; neuron projection; growth cone; lamellipodium; dendrite; apical plasma membrane; plasma membrane; integral to membrane; vacuole; intercellular junction

Molecular Function: arachidonic acid binding; SNAP receptor activity; SNARE binding; protein binding

Biological Process: synaptic vesicle fusion to presynaptic membrane; intracellular protein transport; positive regulation of cell adhesion; vesicle docking; positive regulation of chemotaxis; positive regulation of cell proliferation; neurite development

Research Articles on STX3

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Product Notes

The STX3 stx3 (Catalog #AAA625569) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STX3, ID (Syntaxin-3, STX3A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STX3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the STX3 stx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STX3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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