Rabbit anti-Human STX3 Polyclonal Antibody | anti-STX3 antibody

STX3, ID (Syntaxin-3, STX3A) (Biotin)

Cat. Num. AAA6501857

• Gene Names: STX3; STX3A
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: STX3; Polyclonal Antibody; ID (Syntaxin-3; STX3A) (Biotin); anti-STX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STX3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-STX3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human STX3 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with STX3 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of anti-STX3 Antibody (Center) in A375 cell line lysates (35ug/lane). STX3(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of STX3 (arrow) using rabbit polyclonal STX3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STX3 gene.)

Product Categories/Family for anti-STX3 antibody

Antibodies; Syntaxin; Synaptotagmin; Synaptophysin

References

1. Darios, F., Nature 440(7085), 813-817 (2006). 2. Low, S.H., Mol. Biol. Cell 17(2), 977-989 (2006). 3. Morgans, C.W., J. Neurosci. 16(21), 6713-6721 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4759184
• NCBI GeneID: 6809
• NCBI Accession #: NP_004168
• NCBI GenBank Nucleotide #: NM_004177.4
• UniProt Accession #: Q13277; O43750; O43751; Q15360; B4DME0
• Molecular Weight: 33.2kDa (288aa), confirmed by MALDI-TOF
• NCBI Official Full Name: syntaxin-3 isoform 1
• NCBI Official Synonym Full Names: syntaxin 3
• NCBI Official Symbol: STX3
• NCBI Official Synonym Symbols: STX3A
• NCBI Protein Information: syntaxin-3
• UniProt Protein Name: Syntaxin-3
• Protein Family: Syntaxin
• UniProt Gene Name: STX3
• UniProt Synonym Gene Names: STX3A
• UniProt Entry Name: STX3_HUMAN

NCBI Description

The gene is a member of the syntaxin family. The encoded protein is targeted to the apical membrane of epithelial cells where it forms clusters and is important in establishing and maintaining polarity necessary for protein trafficking involving vesicle fusion and exocytosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

STX3: Potentially involved in docking of synaptic vesicles at presynaptic active zones. Belongs to the syntaxin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: SNARE complex; synaptic vesicle; specific granule; azurophil granule; neuron projection; growth cone; lamellipodium; apical plasma membrane; dendrite; plasma membrane; integral to membrane; vacuole; intercellular junction

Molecular Function: arachidonic acid binding; SNAP receptor activity; protein binding; SNARE binding

Biological Process: synaptic vesicle fusion to presynaptic membrane; intracellular protein transport; vesicle docking; positive regulation of cell adhesion; positive regulation of chemotaxis; positive regulation of cell proliferation; neurite development