Rabbit ZAP 70 Polyclonal Antibody | anti-Zap70 antibody

ZAP 70, phosphorylated (Tyr319) (Tyrosine-protein Kinase ZAP-70, 70kD zeta-associated Protein, Syk-related Tyrosine Kinase, SRK, ZAP70)

Cat. Num. AAA625553

• Gene Names: Zap70; Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ZAP 70; Polyclonal Antibody; phosphorylated (Tyr319) (Tyrosine-protein Kinase ZAP-70; 70kD zeta-associated Protein; Syk-related Tyrosine Kinase; SRK; ZAP70); Anti -ZAP 70; anti-Zap70 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZAP 70 when phosphorylated at (Tyr319). Species Cross-Reactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Zap70 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot:1:500-1:1,000
• Immunogen: Synthetic phosphopeptide corresponding to human Zap-70 around the phosphorylation site of tyrosine 319 (S-P-YP-S-D).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Zap70 antibody

ZAP70, a 70kD member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.

Product Categories/Family for anti-Zap70 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 436480
• NCBI GeneID: 22637
• UniProt Accession #: P43404; P97455; Q80VV2; Q8CHJ3
• Molecular Weight: 70,058 Da
• NCBI Official Full Name: ZAP-70
• NCBI Official Synonym Full Names: zeta-chain (TCR) associated protein kinase
• NCBI Official Symbol: Zap70
• NCBI Official Synonym Symbols: Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364
• NCBI Protein Information: tyrosine-protein kinase ZAP-70; syk-related tyrosine kinase; 70 kDa zeta-associated protein
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Zap70
• UniProt Synonym Gene Names: Srk; Zap-70
• UniProt Entry Name: ZAP70_MOUSE

NCBI Description

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: EC 2.7.10.2; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; membrane; cytoplasm; plasma membrane; immunological synapse; intracellular; intercellular junction; cytosol; lipid raft

Molecular Function: transferase activity; protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; receptor binding; protein kinase activity

Biological Process: thymic T cell selection; cell migration; peptidyl-tyrosine phosphorylation; immune system process; protein amino acid autophosphorylation; positive regulation of calcium-mediated signaling; protein amino acid phosphorylation; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of T cell differentiation; innate immune response; beta selection; immune response; positive regulation of alpha-beta T cell proliferation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive thymic T cell selection