Sheep anti-Mouse IL22RA2 Polyclonal Antibody | anti-IL22RA2 antibody
IL22RA2 (Interleukin-22 Receptor Subunit alpha-2, IL-22 Receptor Subunit alpha-2, IL-22R-alpha-2, IL-22RA2, Cytokine Receptor Family Type 2, Soluble 1, CRF2-S1, Interleukin-22-binding Protein, IL-22BP, IL22BP, ZcytoR16) (APC)
Gene Names
IL22RA2; CRF2X; CRF2-10; CRF2-S1; IL-22BP; IL-22RA2; MGC150509; MGC150510
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
IL22RA2; Polyclonal Antibody; IL22RA2 (Interleukin-22 Receptor Subunit alpha-2; IL-22 Receptor Subunit alpha-2; IL-22R-alpha-2; IL-22RA2; Cytokine Receptor Family Type 2; Soluble 1; CRF2-S1; Interleukin-22-binding Protein; IL-22BP; IL22BP; ZcytoR16) (APC); Anti -IL22RA2 (Interleukin-22 Receptor Subunit alpha-2; anti-IL22RA2 antibody
Host
Sheep
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse IL22RA2.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-IL22RA2 antibody
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels 5x10e5 cells. For intracellular staining, cells must first be fixed with 4% paraformaldehyde fixation buffer and permeabilized with 0.1% saponin balanced salt solution.
Flow Cytometry Protocol: 1. Harvest cells and wash twice in saline buffer.
2. Cell surface staining may be done at this point following the manufacturer's staining procedure.
3. Resuspend 5x10e5 cells in 0.5ml of cold 4% paraformldehyde fixation buffer and incubate at RT for 10 mins.
4. Following fixation, wash the cells twice in saline buffer, then once in 0.1% saponin saline buffer.
5. After permeabilization, add 10ul of I8443-21A and incubate the cells for 30 minutes at RT in the dark.
6. Wash the cells twice with 0.1% saponin saline buffer.
7. Resuspend the cells in saline buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled sheep IgG antibody.
Dilution: Flow Cytometry: 10ul labels 5x10e5 cells. For intracellular staining, cells must first be fixed with 4% paraformaldehyde fixation buffer and permeabilized with 0.1% saponin balanced salt solution.
Flow Cytometry Protocol: 1. Harvest cells and wash twice in saline buffer.
2. Cell surface staining may be done at this point following the manufacturer's staining procedure.
3. Resuspend 5x10e5 cells in 0.5ml of cold 4% paraformldehyde fixation buffer and incubate at RT for 10 mins.
4. Following fixation, wash the cells twice in saline buffer, then once in 0.1% saponin saline buffer.
5. After permeabilization, add 10ul of I8443-21A and incubate the cells for 30 minutes at RT in the dark.
6. Wash the cells twice with 0.1% saponin saline buffer.
7. Resuspend the cells in saline buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled sheep IgG antibody.
Immunogen
Recombinant corresponding to aa19-230 from mouse IL22RA2 expressed in NSO cells (Accession #Q80XF5 with D149E substitution).
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for at 12 months at 4 degree C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.
Related Product Information for anti-IL22RA2 antibody
IL22RA2 is a 40kD secreted glycoprotein that belongs to the class II cytokine receptor family. The mature region contains two fibronectin type III domains. IL22RA2 will bind to an IL-22 dimer, rendering it inactive. The amino acid sequence of mature mouse IL22RA2 is 85% and 67% identical to that of rat and human IL22RA2, respectively. Unlike the human IL22RA2 gene that shows three alternate splice forms, the mouse IL22RA2 gene shows only single nucleotide polymorphisms.
Product Categories/Family for anti-IL22RA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,550 Da
NCBI Official Full Name
interleukin-22 receptor subunit alpha-2 isoform 1
NCBI Official Synonym Full Names
interleukin 22 receptor, alpha 2
NCBI Official Symbol
IL22RA2
NCBI Official Synonym Symbols
CRF2X; CRF2-10; CRF2-S1; IL-22BP; IL-22RA2; MGC150509; MGC150510
NCBI Protein Information
interleukin-22 receptor subunit alpha-2; zcytoR16; IL-22R-alpha-2; OTTHUMP00000017282; OTTHUMP00000017283; OTTHUMP00000017284; class II cytokine receptor; IL-22 receptor subunit alpha-2; interleukin 22-binding protein; interleukin-22-binding protein; cytokine receptor class-II member 10; cytokine receptor family 2 member 10; cytokine receptor family type 2, soluble 1
UniProt Protein Name
Interleukin-22 receptor subunit alpha-2
Protein Family
UniProt Gene Name
IL22RA2
UniProt Entry Name
I22R2_HUMAN
NCBI Description
The protein encoded by this gene is a soluble class II cytokine receptor. This protein has been shown to specifically bind to interleukin 22 (IL22), block the interaction of IL22 with its cell surface receptor, and thus inhibit IL22 activity. This protein functions as an IL22 antagonist, and may be important in the regulation of inflammatory response. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]
Uniprot Description
IL22RA2: Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses. Belongs to the type II cytokine receptor family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 6q23.3
Cellular Component: extracellular space; plasma membrane; integral to membrane; cytosol
Molecular Function: interleukin-22 binding; interleukin-22 receptor activity
Biological Process: regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of inflammatory response; cytokine and chemokine mediated signaling pathway
Research Articles on IL22RA2
Similar Products
Product Notes
The IL22RA2 il22ra2 (Catalog #AAA624576) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The IL22RA2 (Interleukin-22 Receptor Subunit alpha-2, IL-22 Receptor Subunit alpha-2, IL-22R-alpha-2, IL-22RA2, Cytokine Receptor Family Type 2, Soluble 1, CRF2-S1, Interleukin-22-binding Protein, IL-22BP, IL22BP, ZcytoR16) (APC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL22RA2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: 10ul labels 5x10e5 cells. For intracellular staining, cells must first be fixed with 4% paraformaldehyde fixation buffer and permeabilized with 0.1% saponin balanced salt solution. Flow Cytometry Protocol: 1. Harvest cells and wash twice in saline buffer. 2. Cell surface staining may be done at this point following the manufacturer's staining procedure. 3. Resuspend 5x10e5 cells in 0.5ml of cold 4% paraformldehyde fixation buffer and incubate at RT for 10 mins. 4. Following fixation, wash the cells twice in saline buffer, then once in 0.1% saponin saline buffer. 5. After permeabilization, add 10ul of I8443-21A and incubate the cells for 30 minutes at RT in the dark. 6. Wash the cells twice with 0.1% saponin saline buffer. 7. Resuspend the cells in saline buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled sheep IgG antibody. Researchers should empirically determine the suitability of the IL22RA2 il22ra2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL22RA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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