Mouse anti-Human HSP27 Monoclonal Antibody | anti-HSP27 antibody
HSP27 (Heat Shock Protein 27, Heat shock 27kD protein, Estrogen-regulated 24kDa protein, Heat shock 25kD protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)) (BSA & Azide Free)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot, Immunofluorescence, Immunoprecipitation
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
HSP27; Monoclonal Antibody; HSP27 (Heat Shock Protein 27; Heat shock 27kD protein; Estrogen-regulated 24kDa protein; Heat shock 25kD protein 1; Heat shock 28kDa protein 1; Heat shock protein beta-1; HMN2B; Hsp25; Hsp28; HspB1; Stress-responsive protein 27 (SRP27)) (BSA & Azide Free); Heat Shock Protein 27; Heat shock 27kDa protein; Heat shock 25kDa protein 1; Stress-responsive protein 27 (SRP27); anti-HSP27 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
G3.1
Specificity
Recognizes human HSP27. Species Crossreactivity: chimpanzee, sheep, rat, mouse, and chicken.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-HSP27 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC) Frozen/Paraffin, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
FC/FACS: 0.5-1ug/million cells
IHC-F/P: 0.5-1ug/ml for 30 minutes at RT
IP: 0.5-1ug/500ug protein lysate
IF: 0.5-1ug/ml
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
IHC-F/P: 0.5-1ug/ml for 30 minutes at RT
IP: 0.5-1ug/500ug protein lysate
IF: 0.5-1ug/ml
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
Immunogen
Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells. Cellular Localization: Predominantly cytoplasmic with some nuclear
Conjugate
MaxLight750
Positive Control
~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Product Categories/Family for anti-HSP27 antibody
References
1. Edwards DP et. al. Biochem Biophys Research Commun, 93:804-812, 1980. 2. Ciocca DR et. al. Breast Cancer Research and Treatment, 20:33-42, 1991.
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Product Notes
The HSP27 (Catalog #AAA6236498) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSP27 (Heat Shock Protein 27, Heat shock 27kD protein, Estrogen-regulated 24kDa protein, Heat shock 25kD protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)) (BSA & Azide Free) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSP27 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC) Frozen/Paraffin, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). FC/FACS: 0.5-1ug/million cells IHC-F/P: 0.5-1ug/ml for 30 minutes at RT IP: 0.5-1ug/500ug protein lysate IF: 0.5-1ug/ml WB: 0.5-1ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HSP27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP27, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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