Rabbit Nicastrin Polyclonal Antibody | anti-NCSTN antibody

Nicastrin

Cat. Num. AAA623269

• Gene Names: NCSTN; APH2; KIAA0253; RP11-517F10.1
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: Nicastrin; Polyclonal Antibody; Anti -Nicastrin; anti-NCSTN antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human Nicastrin at 110 and 120kD. Species Crossreactivity: mouse, monkey and rat.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-NCSTN antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000 incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
• Immunogen: Synthetic peptide corresponding to residues surrounding the C-terminus of human Nicastrin (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from human (HeLa and SK-N-MC; lanes 1 and 2), monkey (COS; lane 3), mouse (Neuro 2A and L; lanes 4 and 5) and rat (PC12 and C6; lanes 6 and 7) cell lines, using MBS623269.)

Related Product Information for anti-NCSTN antibody

Nicastrin is a transmembrane glycoprotein serving as an essential component of the gammasecretase complex (1,2). Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound gamma-secretase complex (3). Nicastrin also serves as a docking site for gamma-secretase substrates such as APP and Notch, by directly binding to them and presenting them properly to gammasecretase to ensure the correct cleavage process (2,4).

Product Categories/Family for anti-NCSTN antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 9992878
• NCBI GeneID: 23385
• UniProt Accession #: Q5T208; Q5T209; Q92542
• Molecular Weight: 78,411 Da
• NCBI Official Full Name: nicastrin
• NCBI Official Synonym Full Names: nicastrin
• NCBI Official Symbol: NCSTN
• NCBI Official Synonym Symbols: APH2; KIAA0253; RP11-517F10.1
• NCBI Protein Information: nicastrin; ATAG1874; OTTHUMP00000031839; OTTHUMP00000031840; anterior pharynx-defective 2
• UniProt Protein Name: Nicastrin
• Protein Family: Nicastrin
• UniProt Gene Name: NCSTN
• UniProt Entry Name: Q5T208_HUMAN

NCBI Description

This gene encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined. [provided by RefSeq]

Uniprot Description

nicastrin: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP). Constitutively expressed in neural cells. Widely expressed. Belongs to the nicastrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22-q23

Cellular Component: Golgi apparatus; focal adhesion; membrane; lysosomal membrane; endoplasmic reticulum; integral to plasma membrane; integral to membrane; melanosome; plasma membrane

Molecular Function: protein binding; endopeptidase activity

Biological Process: positive regulation of catalytic activity; axon guidance; Notch signaling pathway; extracellular matrix organization and biogenesis; membrane protein intracellular domain proteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; myeloid cell homeostasis; membrane protein ectodomain proteolysis; Notch receptor processing; proteolysis; T cell proliferation; extracellular matrix disassembly; amyloid precursor protein catabolic process; epithelial cell proliferation; beta-amyloid metabolic process; ephrin receptor signaling pathway; protein processing

Disease: Acne Inversa, Familial, 1