Goat BAG6/G3/Scythe Polyclonal Antibody | anti-BAG6 antibody

Anti-BAG6/G3/Scythe Antibody (aa257-270)

Cat. Num. AAA2400838

• Gene Names: BAG6; G3; BAT3; BAG-6; D6S52E
• Reactivity: Human; Predicted: Dog, Bovine, Pig, Monkey
• Applications: ELISA, Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: BAG6/G3/Scythe; Polyclonal Antibody; Anti-BAG6/G3/Scythe Antibody (aa257-270); Rabbit Polyclonal to Mouse BAG6/G3/Scythe; BAG6; BCL2-associated athanogene 6; D6S52E; HLA-B associated transcript 3; HLA-B-associated transcript 3; HLA-B associated transcript-3; G3; Protein G3; Protein Scythe; Scythe; BAG-6; BAT3; anti-BAG6 antibody

• Host: Goat
• Reactivity: Human; Predicted: Dog, Bovine, Pig, Monkey
• Clonality: Polyclonal
• Specificity: Human BAG6/BAT3. This antibody is expected to recognize both reported isoforms (NP_004630.3; NP_542433.1). Reported variants representing identical protein: NP_542434.1, NP_001092004.1, NP_542433.1 (isoform b).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Sequence Length: 1132

• Applicable Applications for anti-BAG6 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: 1:128000; IHC-P: 3.75 ug/ml
• Immunogen: Synthetic peptide C-PETNAPNHPSPAEY from an internal region of human BAG6/BAT3 (NP_004630.3; NP_542433.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bovine, Pig (100%); Mouse, Rat, Hamster, Bat, Horse (93%).
• Conjugation: Unconjugated
• Epitope: aa257-270
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-BAG6/G3/Scythe antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-BAG6 antibody

Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmicreticulum membrane and regulation of chromatin. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also namedtype II transmembrane proteins, contain a single C-terminaltransmembrane region.

NCBI and Uniprot Product Information

• NCBI GI #: 149158692
• NCBI GeneID: 7917
• NCBI Accession #: NP_004630.3
• NCBI GenBank Nucleotide #: NM_004639.3
• UniProt Accession #: P46379; O95874; A2ADJ7; A3KQ42; A3KQ44; A6NGY6; A6PWF7; B0UX84; B4DZ12; B4E3V4; E7EMZ4; F8VXY4
• Molecular Weight: 113,456 Da
• NCBI Official Full Name: large proline-rich protein BAG6 isoform a
• NCBI Official Synonym Full Names: BCL2 associated athanogene 6
• NCBI Official Symbol: BAG6
• NCBI Official Synonym Symbols: G3; BAT3; BAG-6; D6S52E
• NCBI Protein Information: large proline-rich protein BAG6
• UniProt Protein Name: Large proline-rich protein BAG6
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG6
• UniProt Synonym Gene Names: BAG-6

NCBI Description

This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP-independent molecular chaperone preventing the aggregation of misfolded and hydrophobic patches-containing proteins (PubMed:21636303). Functions as part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, which maintains these client proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20516149, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20516149, PubMed:20676083, PubMed:28104892). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated by RNF126, an E3 ubiquitin-protein ligase associated with BAG6 and are sorted to the proteasome (PubMed:24981174, PubMed:28104892, PubMed:27193484). SGTA which prevents the recruitment of RNF126 to BAG6 may negatively regulate the ubiquitination and the proteasomal degradation of client proteins (PubMed:23129660, PubMed:25179605, PubMed:27193484). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303). BAG6 is also required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, it may participate to the production of antigenic peptides and play a role in antigen presentation in immune response (). BAG6 is also involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. BAG6 may ensure the proper degradation of these proteins and thereby protects the endoplasmic reticulum from protein overload upon stress (PubMed:26565908). By inhibiting the polyubiquitination and subsequent proteasomal degradation of HSPA2 it may also play a role in the assembly of the synaptonemal complex during spermatogenesis (). Also positively regulates apoptosis by interacting with and stabilizing the proapoptotic factor AIFM1 (). By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway (PubMed:26692333).