Rabbit DDX48 Polyclonal Antibody | anti-EIF4A3 antibody

DDX48 (DEAD (Asp-Glu-Ala-Asp) Box 48, EIF4a3, eIF4aIII, KIAA0111, MGC10862, NUK-34, hNMP265)

Cat. Num. AAA621561

• Gene Names: EIF4A3; DDX48; NUK34; NMP265; KIAA0111; MGC10862; eIF4AIII; DKFZp686O16189
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Serum; Serum
• Synonyms: DDX48; Polyclonal Antibody; DDX48 (DEAD (Asp-Glu-Ala-Asp) Box 48; EIF4a3; eIF4aIII; KIAA0111; MGC10862; NUK-34; hNMP265); Anti -DDX48 (DEAD (Asp-Glu-Ala-Asp) Box 48; anti-EIF4A3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human DDX48. Species Crossreactivity: mouse, rat.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid, 0.05% sodium azide.

• Applicable Applications for anti-EIF4A3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic linear peptide corresponding to DDX48 (KLH).
• Positive Control: A431 cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Analysis: Representative lot data. A431 cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS621561 (1:1000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MBS621561 (~34kD).)

Western Blot (WB)

(Western Blot Analysis: Representative lot data. A431 (Lane1), HEK293 lysate (Lane 2), HeLa lysate (Lane 3), HepG2 lysate (Lane 4), Jurkat lysate (Lane 5), PC12 lysate ( Lane 6), PC3 lysate (Lane 7) and RAW264.7 lysate (Lane 8) were resolved by electrophoresis, transferred to PVDF membrane and probed with the Ab (1:1000 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MBS621561 (~34kD).)

Related Product Information for anti-EIF4A3 antibody

Eukaryotic translation initiation factor 4A, isoform 3, also known as eIF4A3, is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. eIF4A3 is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.

Product Categories/Family for anti-EIF4A3 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 48145617
• NCBI GeneID: 9775
• UniProt Accession #: P38919; Q15033; Q6IBQ2; Q96A18
• Molecular Weight: 46,871 Da
• NCBI Official Full Name: DDX48
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4A3
• NCBI Official Symbol: EIF4A3
• NCBI Official Synonym Symbols: DDX48; NUK34; NMP265; KIAA0111; MGC10862; eIF4AIII; DKFZp686O16189
• NCBI Protein Information: eukaryotic initiation factor 4A-III; NMP 265; hNMP 265; eIF4A-III; eIF-4A-III; DEAD box protein 48; nuclear matrix protein 265; ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; eukaryotic initiation factor 4A-like NUK-34; eukaryotic translation initiation factor 4A, isoform 3
• UniProt Protein Name: Eukaryotic initiation factor 4A-III
• Protein Family: Eukaryotic initiation factor
• UniProt Gene Name: EIF4A3
• UniProt Synonym Gene Names: DDX48; KIAA0111
• UniProt Entry Name: IF4A3_HUMAN

NCBI Description

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq]

Uniprot Description

DDX48: ATP-dependent RNA helicase. Component of a splicing- dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Belongs to the DEAD box helicase family. eIF4A subfamily.

Protein type: RNA splicing; EC 3.6.4.13; RNA-binding; Helicase; Spliceosome

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; membrane; cytoplasm; nuclear speck; cytosol

Molecular Function: mRNA binding; protein binding; poly(A) binding; ATP-dependent RNA helicase activity; ATP binding

Biological Process: nuclear mRNA splicing, via spliceosome; poly(A) tail shortening; mRNA transport; positive regulation of translation; cytokine and chemokine mediated signaling pathway; negative regulation of translation; mRNA catabolic process, nonsense-mediated decay; gene expression; embryonic cranial skeleton morphogenesis; rRNA processing; mRNA catabolic process, deadenylation-dependent decay

Disease: Robin Sequence With Cleft Mandible And Limb Anomalies