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Testing Data

IgM secondary antibody

Goat F (ab')2 Anti-Mouse IgM, Human ads-HRP

Reactivity
Mouse IgG and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry
Synonyms
IgM; Goat F (ab')2 Anti-Mouse IgM; Human ads-HRP; IgM secondary antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Mouse IgG and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Isotype
IgG
Specificity
Reacts with the heavy chain of mouse IgM
Form/Format
Supplied in a stock solution in 50% glycerol/50% PBS, pH 7.4; No preservative added
Applicable Applications for IgM secondary antibody
ELISA (EIA), Fluorescence-Linked Immunosorbent Assay (FLISA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC)
Volume
1.0 mL
Source Note
Pepsin digest of Goat Anti-Mouse IgM, Human ads
Conjugate
HRP (Horseradish Peroxidase)
Preparation and Storage
Store at 2-8 degree C.

Testing Data

Testing Data
References
1. Kang M, Lu H, Yasui T, Sharpe A, Warren H, Cahir-McFarland E, et al. Epstein-Barr virus nuclear antigen 1 does not induce lymphoma in transgenic FVB mice. Proc Natl Acad Sci USA. 2005;102:820-5. (ELISA). 2. Yan J, Vetvicka V, Xia Y, Coxon A, Carroll MC, Mayadas TN, et al. beta-glucan, a "specific" biologic response modifier that uses antibodies to target tumors for cytotoxic recognition by leukocyte complement receptor type 3 (CD11b/CD18). J Immunol. 1999;163:3045-52. (FC). 3. Stabel TJ, Bolin SR, Pesch BA, Rahner TE. A simple and rapid flow cytometric method for detection of porcine cell surface markers. J Immunol Methods. 2000;245:147-52. (FC). 4. de Baey A, Mende I, Baretton G, Greiner A, Hartl WH, Baeuerle PA, et al. A subset of human dendritic cells in the T cell area of mucosa-associated lymphoid tissue with a high potential to produce TNF-alpha. J Immunol. 2003;170:5089-94. (FC). 5. Wallace DL, Zhang Y, Ghattas H, Worth A, Irvine A, Bennett AR, et al. Direct measurement of T cell subset kinetics in vivo in elderly men and women. J Immunol. 2004;173:1787-94. (FC). 6. Hoffman LM, Hall L, Batten JL, Young H, Pardasani D, Baetge EE, et al. X-inactivation status varies in human embryonic stem cell lines. Stem Cells. 2005;23:1468-78. (FC). 7. Guikema JE, Schrader CE, Leus NG, Ucher A, Linehan EK, Werling U, et al. Reassessment of the role of Mut S homolog 5 in Ig class switch recombination shows lack of involvement in cis- and trans-switching. J Immunol. 2008;181:8450-9. (FC). 8. Geraldes P, Rebrovich M, Herrmann K, Wong J, Jäck H, Wabl M, et al. Ig heavy chain promotes mature B cell survival in the absence of light chain. J Immunol. 2007;179:1659-68. (IHC-FS). 9. van Ginkel FW, Wahl SM, Kearney JF, Kweon M, Fujihashi K, Burrows PD, et al. Partial IgA-deficiency with increased Th2-type cytokines in TGF-beta1 knockout mice. J Immunol. 1999;163:1951-7. (IHC-PS). 10. Montalvo F, Almeida GM, Silva EM, Borges VM, Vasconcellos R, Takiya CM, et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages. Eur J Immunol. 2010;40:417-25. (ICC). 11. Miyazaki A, Yogosawa S, Murakami A, Kitamura D. Identification of CMTM7 as a transmembrane linker of BLNK and the B-cell receptor. PLoS One. 2012;7 (2):e31829. (ICC). 12. Takeshita H, Taniuchi I, Kato J, Watanabe T. Abrogation of autoimmune disease in Lyn-deficient mice by the mutation of the Btk gene. Int Immunol. 1998;10:435-44. (Stim). 13. Oliver AM, Martin F, Kearney JF. IgMhighCD21high lymphocytes enriched in the splenic marginal zone generate effector cells more rapidly than the bulk of follicular B cells. J Immunol. 1999;162:7198-207. (Stim). 14. Ochi H, Watanabe T. Negative regulation of B cell receptor-mediated signaling in B-1 cells through CD5 and Ly49 co-receptors via Lyn kinase activity. Int Immunol. 2000;12:1417-23. (Stim). 15. Kato I, Takai T, Kudo A. The pre-B cell receptor signaling for apoptosis is negatively regulated by FcgammaRIIB. J Immunol. 2002;168:629-34. (Stim). 16. Gavin AL, Aït-Azzouzene D, Ware CF, Nemazee D. deltaBAFF, an alternate splice isoform that regulates receptor binding and biopresentation of the B cell survival cytokine, BAFF. J Biol Chem. 2003;278:38220-8. (Stim). 17. Chattopadhyay G, Khan AQ, Sen G, Colino J, duBois W, Rubtsov A, et al. Transgenic expression of Bcl-xL or Bcl-2 by murine B cells enhances the in vivo antipolysaccharide, but not antiprotein, response to intact Streptococcus pneumoniae. J Immunol. 2007;179:7523-34. (Stim). 18. Bahjat FR, Pine PR, Reitsma A, Cassafer G, Baluom M, Grillo S, et al. An orally bioavailable spleen tyrosine kinase inhibitor delays disease progression and prolongs survival in murine lupus. Arthritis Rheum. 2008;58:1433-44. (Stim). 19. Hauser J, Verma-Gaur J, Wallenius A, Grundström T. Initiation of antigen receptor-dependent differentiation into plasma cells by calmodulin inhibition of E2A. J Immunol. 2009;183:1179-87. (Stim). 20. Woo A, Kim J, Jeong Y, Maeng HG, Lee Y, Kang JS, et al. Vitamin C acts indirectly to modulate isotype switching in mouse B cells. Anat Cell Biol. 2010;43:25-35. (Stim). 21. Hauser J, Grundström C, Grundström T. Allelic exclusion of IgH through inhibition of E2A in a VDJ recombination complex. J Immunol. 2014;192:2460-70. (Stim). 22. Lindner JM, Kayo H, Hedlund S, Fukuda Y, Fukao T, Nielsen PJ. Cutting edge: The transcription factor Bob1 counteracts B cell activation and regulates miR-146a in B cells. J Immunol. 2014;192:4483-6. (Stim). 23. Goverse G, Olivier BJ, Molenaar R, Knippenberg M, Greuter M, Konijn T, et al. Vitamin A metabolism and mucosal immune function are distinct between BALB/c and C57BL/6 mice. Eur J Immunol. 2015;45:89-100. (Stim). 24. Hu J, Oda SK, Shotts K, Donovan EE, Strauch P, Pujanauski LM, et al. Lysophosphatidic acid receptor 5 inhibits B cell antigen receptor signaling and antibody response. J Immunol. 2014;193:85-95. (Stim). 25. Bestas B, Moreno PM, Blomberg KE, Mohammad DK, Saleh AF, Sutlu T, et al. Splice-correcting oligonucleotides restore BTK function in X-linked agammaglobulinemia model. J Clin Invest. 2014;124:4067-81. (Stim)

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Product Notes

The IgM (Catalog #AAA679274) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat F (ab')2 Anti-Mouse IgM, Human ads-HRP reacts with Mouse IgG and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species and may cross-react with other species as described in the data sheet. AAA Biotech's IgM can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Fluorescence-Linked Immunosorbent Assay (FLISA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgM, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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