Rabbit anti-Human EIF4A3 Polyclonal Antibody | anti-EIF4A3 antibody

EIF4A3, CT (EIF4A3, DDX48, KIAA0111, Eukaryotic initiation factor 4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic translation initiation factor 4A i

Cat. Num. AAA6295082

• Gene Names: EIF4A3; RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EIF4A3; Polyclonal Antibody; CT (EIF4A3; DDX48; KIAA0111; Eukaryotic initiation factor 4A-III; ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD box protein 48; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A i; Eukaryotic translation initiation factor 4A isoform 3; Nuclear matrix protein 265; anti-EIF4A3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-EIF4A3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: EIF4A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-325 amino acids from the C-terminal region of human EIF4A3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(EIF4A3 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the EIF4A3 antibody detected the EIF4A3 protein (arrow).)

Related Product Information for anti-EIF4A3 antibody

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.

Product Categories/Family for anti-EIF4A3 antibody

Antibodies; DEAD Box Proteins

References

Koroleva, O.A., et al. Plant Cell 21(5):1592-1606(2009). Karam, R., et al. Oncogene 27(30):4255-4260(2008). Ma, X.M., et al. Cell 133(2):303-313(2008). Giorgi, C., et al. Cell 130(1):179-191(2007). Zhang, Z., et al. Proc. Natl. Acad. Sci. U.S.A. 104(28):11574-11579(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 288915541
• NCBI GeneID: 9775
• NCBI Accession #: NP_055555.1
• NCBI GenBank Nucleotide #: NM_014740
• UniProt Accession #: P38919; Q15033; Q6IBQ2; Q96A18
• Molecular Weight: 46,871 Da
• NCBI Official Full Name: Homo sapiens eukaryotic translation initiation factor 4A3 (EIF4A3), mRNA
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4A3
• NCBI Official Symbol: EIF4A3
• NCBI Official Synonym Symbols: RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII
• NCBI Protein Information: eukaryotic initiation factor 4A-III; ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; NMP 265; eIF-4A-III; eIF4A-III; eukaryotic initiation factor 4A-like NUK-34; eukaryo
• UniProt Protein Name: Eukaryotic initiation factor 4A-III
• Protein Family: Eukaryotic initiation factor
• UniProt Gene Name: EIF4A3
• UniProt Synonym Gene Names: DDX48; KIAA0111; eIF-4A-III; eIF4A-III; NMP 265; hNMP 265
• UniProt Entry Name: IF4A3_HUMAN

NCBI Description

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX48: ATP-dependent RNA helicase. Component of a splicing- dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Belongs to the DEAD box helicase family. eIF4A subfamily.

Protein type: EC 3.6.4.13; Spliceosome; RNA-binding; Helicase; RNA splicing

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; membrane; cytoplasm; nuclear speck; cytosol

Molecular Function: mRNA binding; protein binding; ATP-dependent RNA helicase activity; ATP binding; poly(A) binding

Biological Process: nuclear mRNA splicing, via spliceosome; poly(A) tail shortening; mRNA transport; positive regulation of translation; cytokine and chemokine mediated signaling pathway; negative regulation of translation; mRNA catabolic process, nonsense-mediated decay; gene expression; embryonic cranial skeleton morphogenesis; rRNA processing; mRNA catabolic process, deadenylation-dependent decay

Disease: Robin Sequence With Cleft Mandible And Limb Anomalies