Rabbit Cell division cycle associated 8 Polyclonal Antibody | anti-CDCA8 antibody

Cell division cycle associated 8 (BOR, Borealin, BOREALIN, Cell division cycle-associated protein 8, Dasra-B, FLJ10468, FLJ12042, hDasra-B, PESCRG3, Pluripotent embryonic stem cell-related gene 3 protein)

Cat. Num. AAA620401

• Gene Names: CDCA8; BOR; DasraB; MESRGP; BOREALIN; FLJ10468; FLJ12042
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Cell division cycle associated 8; Polyclonal Antibody; Cell division cycle associated 8 (BOR; Borealin; BOREALIN; Cell division cycle-associated protein 8; Dasra-B; FLJ10468; FLJ12042; hDasra-B; PESCRG3; Pluripotent embryonic stem cell-related gene 3 protein); Anti -Cell division cycle associated 8 (BOR; anti-CDCA8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human CDCA8. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS containing 0.02% sodium azide.

• Applicable Applications for anti-CDCA8 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: Synthetic peptide corresponding to 19aa near the amino terminus of the human CDCA8.
• Positive Control: Rat Kidney Tissue Lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of CDCA8 in Rat kidney lysate with CDCA8 antibody at (A) 1 and (B) 2 ug/ml.)

Related Product Information for anti-CDCA8 antibody

CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.

Product Categories/Family for anti-CDCA8 antibody

Antibodies; Abs to Cell Division Cycle (CDC)

NCBI and Uniprot Product Information

• NCBI GI #: 16877392
• NCBI GeneID: 55143
• UniProt Accession #: Q53HL2; Q53HN1; Q96AM3; Q9NVW5; D3DPT4
• Molecular Weight: 31,323 Da
• NCBI Official Full Name: Cell division cycle associated 8
• NCBI Official Synonym Full Names: cell division cycle associated 8
• NCBI Official Symbol: CDCA8
• NCBI Official Synonym Symbols: BOR; DasraB; MESRGP; BOREALIN; FLJ10468; FLJ12042
• NCBI Protein Information: borealin; Dasra B; dasra-B; hDasra-B; OTTHUMP00000004514; OTTHUMP00000004515; cell division cycle-associated protein 8; pluripotent embryonic stem cell-related gene 3 protein
• UniProt Protein Name: Borealin
• UniProt Gene Name: CDCA8
• UniProt Synonym Gene Names: PESCRG3
• UniProt Entry Name: BOREA_HUMAN

NCBI Description

CDCA8 is a component of a chromosomal passenger complex required for stability of the bipolar mitotic spindle (Gassmann et al., 2004 [PubMed 15249581]).[supplied by OMIM]

Uniprot Description

Borealin: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Belongs to the borealin family.

Protein type: Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: intercellular bridge; protein complex; nucleolus; spindle; midbody; cytosol; nucleus; chromosome, pericentric region; chromocenter

Molecular Function: protein binding

Biological Process: cell division; mitotic cell cycle; mitotic metaphase plate congression; chromosome organization and biogenesis