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Western Blot (WB) (Fig.1. Western Blot analysis of 453(1), AD293(2), diluted at 1:2000.)

Rabbit PERK Polyclonal Antibody | anti-PERK antibody

PERK (Phospho Thr981) Polyclonal Antibody

Gene Names
EIF2AK3; PEK; WRS; PERK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
PERK; Polyclonal Antibody; PERK (Phospho Thr981) Polyclonal Antibody; Rabbit Anti-PERK (phospho Thr981) Polyclonal Antibody; EIF2AK3; PEK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; anti-PERK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-PERK (T981) Polyclonal Antibody detects endogenous levels of PERK protein only when phosphorylated at T981.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1116
Applicable Applications for anti-PERK antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry Paraffin (IHC-P), ELISA (EIA)
Application Notes
IHC-P: 1:100-1:300
ELISA: 1:40000
Optimal dilutions to be determined by enduser.
Immunogen
Synthesized peptide derived from human PERK around the phosphorylation site of T981
Note
Contain sodium azide.
Preparation and Storage
Store at -20°C. Avoid repeated freeze/thaw cycles.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Fig.1. Western Blot analysis of 453(1), AD293(2), diluted at 1:2000.)

Western Blot (WB) (Fig.1. Western Blot analysis of 453(1), AD293(2), diluted at 1:2000.)

Immunofluorescence (IF)

(Fig.2. Immunofluorescence analysis of rat heart tissue. 1, PERK (phospho Thr981) Polyclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)

Immunofluorescence (IF) (Fig.2. Immunofluorescence analysis of rat heart tissue. 1, PERK (phospho Thr981) Polyclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Related Product Information for anti-PERK antibody
Eukaryotic translation initiation factor 2 alpha kinase 3 encoded by EIF2AK3 phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Eukaryotic translation initiation factor 2 alpha kinase 3 is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in EIF2AK3 are associated with Wolcott-Rallison syndrome.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2 alpha kinase 3
NCBI Official Symbol
EIF2AK3
NCBI Official Synonym Symbols
PEK; WRS; PERK
NCBI Protein Information
eukaryotic translation initiation factor 2-alpha kinase 3
UniProt Protein Name
Eukaryotic translation initiation factor 2-alpha kinase 3
UniProt Gene Name
EIF2AK3
UniProt Synonym Gene Names
PEK; PERK; HsPEK
UniProt Entry Name
E2AK3_HUMAN

NCBI Description

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

Uniprot Description

PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK.

Protein type: EC 2.7.11.1; Membrane protein, integral; Translation; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; PEK family; PEK subfamily

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytoplasm; integral to endoplasmic reticulum membrane

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; enzyme binding; protein phosphatase binding; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity

Biological Process: fat cell differentiation; lactation; translation; positive regulation of protein binding; protein amino acid autophosphorylation; protein amino acid phosphorylation; bone mineralization; positive regulation of transcription from RNA polymerase I promoter; cellular response to glucose starvation; regulation of fatty acid metabolic process; angiogenesis; skeletal development; ER overload response; protein homooligomerization; caspase activation; endoplasmic reticulum organization and biogenesis; ossification; negative regulation of myelination; unfolded protein response; calcium-mediated signaling; chondrocyte development; SREBP-mediated signaling pathway; endocrine pancreas development; peptidyl-serine phosphorylation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; insulin-like growth factor receptor signaling pathway; insulin secretion; negative regulation of translation; negative regulation of translation initiation in response to stress; negative regulation of apoptosis

Disease: Epiphyseal Dysplasia, Multiple, With Early-onset Diabetes Mellitus

Research Articles on PERK

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Product Notes

The PERK eif2ak3 (Catalog #AAA9701266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PERK (Phospho Thr981) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PERK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry Paraffin (IHC-P), ELISA (EIA). IHC-P: 1:100-1:300 ELISA: 1:40000 Optimal dilutions to be determined by enduser. Researchers should empirically determine the suitability of the PERK eif2ak3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PERK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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