Rabbit CDCA8 Polyclonal Antibody | anti-CDCA8 antibody

CDCA8 Antibody

Cat. Num. AAA151409

• Gene Names: CDCA8; BOR; DasraB; MESRGP; BOREALIN
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: CDCA8 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CDCA8; Polyclonal Antibody; CDCA8 Antibody; BOR; DasraB; MESRGP; BOREALIN; PESCRG3; Borealin; Cell division cycle-associated protein 8; hDasra-B; cell division cycle associated 8; anti-CDCA8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: CDCA8 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 280

• Applicable Applications for anti-CDCA8 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: CDCA8 antibody can be used for detection of CDCA8 by Western blot at 1 and 2 mug/mL. Antibody can also be used for immunoflourescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CDCA8 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of the human CDCA8.
• Buffer: CDCA8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CDCA8 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CDCA8 in Rat kidney lysate with CDCA8 antibody at (A) 1 and (B) 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of CDCA8 in rat kidney tissue with CDCA8 antibody at 20 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of CDCA8 in Rat Kidney cells with CDCA8 antibody at 20 μg/mL.)

Related Product Information for anti-CDCA8 antibody

CDCA8 Antibody: CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 8922438
• NCBI GeneID: 55143
• NCBI Accession #: NP_060571
• NCBI GenBank Nucleotide #: NM_018101.3
• UniProt Accession #: Q53HL2; Q53HN1; Q96AM3; Q9NVW5; D3DPT4
• Molecular Weight: 31,323 Da
• NCBI Official Full Name: borealin
• NCBI Official Synonym Full Names: cell division cycle associated 8
• NCBI Official Symbol: CDCA8
• NCBI Official Synonym Symbols: BOR; DasraB; MESRGP; BOREALIN
• NCBI Protein Information: borealin; Dasra B; dasra-B; hDasra-B; cell division cycle-associated protein 8; pluripotent embryonic stem cell-related gene 3 protein
• UniProt Protein Name: Borealin
• UniProt Gene Name: CDCA8
• UniProt Synonym Gene Names: PESCRG3; hDasra-B
• UniProt Entry Name: BOREA_HUMAN

NCBI Description

This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013]

Uniprot Description

Borealin: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Belongs to the borealin family.

Protein type: Cell cycle regulation; Nucleolus

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: intercellular bridge; protein complex; nucleolus; spindle; midbody; nucleus; cytosol; chromosome, pericentric region; chromocenter

Molecular Function: protein binding

Biological Process: cell division; mitotic cell cycle; mitotic metaphase plate congression; chromosome organization and biogenesis