Rabbit Elastin, Ligamentum Nuchae Polyclonal Antibody | anti-TMEM43 antibody
Elastin, Ligamentum Nuchae
Gene Names
TMEM43; MGC165694
Reactivity
Bovine, Canine, Human, Rabbit
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
Purity
SerumSerum
Synonyms
Elastin; Ligamentum Nuchae; Polyclonal Antibody; Anti -Elastin; anti-TMEM43 antibody
Host
Rabbit
Reactivity
Bovine, Canine, Human, Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes insoluble Elastin. Reacts with human tropoelastin. Species Crossreactivity: human, bovine, rabbit, canine. Shows little if any reactivity with rat elastin.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a lyophilized powder. Reconstitute with 500ul sterile ddH2O.
Applicable Applications for anti-TMEM43 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Dilution: Western Blot: 1:200-1:500
ELISA: 1:1000-1:2000
Immunoprecipitation: 1:100-1:200
Immunohistochemistry: 1:50-1:100
Immunocytochemistry: 1:50-1:100
Note: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
ELISA: 1:1000-1:2000
Immunoprecipitation: 1:100-1:200
Immunohistochemistry: 1:50-1:100
Immunocytochemistry: 1:50-1:100
Note: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
Immunogen
Alpha-elastin fraction of insoluble elastin purified by hot sodium hydroxide.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-TMEM43 antibody
Elastin is a protein in connective tissue and allows many tissues in the body to resume their shape after stretching or contracting. Elastin helps skin to return to its original position when it is poked or pinched. Elastin is also an important load-bearing tissue in the bodies of mammals and used in places where mechanical energy is required to be stored. Elastin is particularly abundant in large elastic blood vessels such as the aorta. It is also very important in the lungs, elastic ligaments, the skin, the bladder and elastic cartilage. Elastin is composed largely of glycine, proline and other hydrophobic residues and contains multiple lysine-derived crosslinks. The hydrophobic regions of the chains, between the crosslinks, are highly mobile. The hydrophobic and crosslinking domains are coded by separate, small (27-114bp) exons that are separated by large introns. The initial translation product is a 72kD polypeptide, designated tropoelastin. Elastin is made by linking many soluble tropoelastin protein molecules, in a reaction catalyzed by lysyl oxidase, to make a massive insoluble, durable cross-linked array.
Product Categories/Family for anti-TMEM43 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
3,809 Da
NCBI Official Full Name
elastin
NCBI Official Symbol
TMEM43
NCBI Official Synonym Symbols
MGC165694
NCBI Protein Information
transmembrane protein 43; elastin
UniProt Protein Name
Elastin
Protein Family
UniProt Entry Name
Q28102_BOVIN
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Product Notes
The TMEM43 (Catalog #AAA619141) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Elastin, Ligamentum Nuchae reacts with Bovine, Canine, Human, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Elastin, Ligamentum Nuchae can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Dilution: Western Blot: 1:200-1:500 ELISA: 1:1000-1:2000 Immunoprecipitation: 1:100-1:200 Immunohistochemistry: 1:50-1:100 Immunocytochemistry: 1:50-1:100 Note: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols. Researchers should empirically determine the suitability of the TMEM43 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Elastin, Ligamentum Nuchae, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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