Rabbit FZD8 Polyclonal Antibody | anti-FZD8 antibody

FZD8 Antibody

Cat. Num. AAA9404713

• Gene Names: FZD8; FZ-8; hFZ8
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: FZD8; Polyclonal Antibody; FZD8 Antibody; FZ-8; hFZ8; anti-FZD8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total FZD8 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 1.1 mg/ml (varies by lot)
• Sequence Length: 694

• Applicable Applications for anti-FZD8 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:25-1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to residues near the C terminal of human frizzled family receptor 8
• Target Name: FZD8
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/30.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/30.)

Related Product Information for anti-FZD8 antibody

This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.

Product Categories/Family for anti-FZD8 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 13994190
• NCBI GeneID: 8325
• NCBI Accession #: NP_114072.1
• NCBI GenBank Nucleotide #: NM_031866.2
• UniProt Accession #: Q9H461
• Molecular Weight: 73,300 Da
• NCBI Official Full Name: frizzled-8
• NCBI Official Synonym Full Names: frizzled class receptor 8
• NCBI Official Symbol: FZD8
• NCBI Official Synonym Symbols: FZ-8; hFZ8
• NCBI Protein Information: frizzled-8
• UniProt Protein Name: Frizzled-8
• Protein Family: Frizzled
• UniProt Gene Name: FZD8
• UniProt Synonym Gene Names: Fz-8; hFz8
• UniProt Entry Name: FZD8_HUMAN

NCBI Description

This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD8: Receptor for Wnt proteins. Component of the Wnt-Fzd- LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome- sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, Fz/Smo family

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: Golgi apparatus; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding; ubiquitin protein ligase binding; PDZ domain binding; receptor binding

Biological Process: neuron differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of JNK activity; T cell differentiation in the thymus; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter