anti-Human Mst4 Polyclonal Antibody | anti-Mst4 antibody

Anti - Mst4 (Mammalian Ste20-like Kinase)

Cat. Num. AAA617679

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by protein A and peptide affinity chromatography.
• Synonyms: Mst4; Polyclonal Antibody; Anti - Mst4 (Mammalian Ste20-like Kinase); Mst4 (Mammalian Ste20-like Kinase); anti-Mst4 antibody

• Host: Host: Rabbit; Source: Human
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total Mst4 protein independent of phosphorylation. Does not crossreact with Mst1-3.
• Purity/Purification: Purified by protein A and peptide affinity chromatography.
• Form/Format: Affinity Purified; Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
• Sequence Length: 416

• Applicable Applications for anti-Mst4 antibody: Western Blot
• Application Notes: Suitable for use in Western Blotting. Other applications not tested.; Dilution: Western Blot: 1:1000; Optimal dilutions to be determined by the researcher.
• Immunogen: Synthetic (KLH coupled) corresponding to the N-terminal residues of human Mst4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using Mst4 Antibody MBS617679.)

Related Product Information for anti-Mst4 antibody

Mammalian Ste20-like kinases, including Mst1-4, belong to the germinal center kinase (GCK) family. The N-terminal kinase domain of Mst is considerably homologous to the kinase domain of yeast Ste20 kinase and other p21-activated kinases (1). The C-terminal region of Mst1 and Mst2 contains a dimerization and an inhibitory domain, while the C-terminal region of Mst3 and Mst4 are not well characterized (1-3). Dimerization and phosphorylation at the activation loop regulates translocation of Mst1 from the cytosol to the nucleus (3). Growing evidence indicates that Mst1 and Mst2 are activated by apoptotic signals as well as other stress conditions (4-6). The full activation of Mst1 requires both phosphorylation and caspase-mediated cleavage (4). Activated Mst kinases may rely on p38 MAPK and JNK pathways to amplify apoptotic signals (5). Inhibitory phosphorylation is also reported. Phosphorylation at serine 327 of Mst1, which is close to the caspase-3 recognization site, inhibits caspasemediated cleavage (4).

Product Categories/Family for anti-Mst4 antibody

Abs to Protein Kinases

References

• 1. Dan, I. et al. (2001) Trends Cell Biol. 11, 220-230.
• 2. Creasy, C.L. et al. (1996) J. Biol. Chem. 271, 21049-21053.
• 3. Lee, K. and Yonehara, S. (2002) J. Biol. Chem. 277, 12351-12358.
• 4. Graves, J.D. et al. (2001) J. Biol. Chem. 276, 14909-14915.
• 5. Lee, K. et al. (2001) J. Biol. Chem. 276, 19276-19285.
• 6. Graves, J.D. et al. (1998) EMBO J. 17, 2224-2234.

NCBI and Uniprot Product Information

• NCBI GI #: 158455129
• NCBI GeneID: 539445
• UniProt Accession #: Q08DU5
• Molecular Weight: 46,585 Da
• NCBI Official Full Name: MST4 protein
• NCBI Official Symbol: MST4
• NCBI Protein Information: serine/threonine-protein kinase MST4
• UniProt Protein Name: MST4 protein
• UniProt Gene Name: MST4
• UniProt Entry Name: Q08DU5_BOVIN