Mouse anti-Human BCL9 Monoclonal Antibody | anti-BCL9 antibody

BCL9 (B Cell CLL/Lymphoma 9 Protein, B Cell Lymphoma 9 Protein, Bcl-9, Protein Legless Homolog) (FITC)

Cat. Num. AAA6146116

• Gene Names: BCL9; LGS
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BCL9; Monoclonal Antibody; BCL9 (B Cell CLL/Lymphoma 9 Protein; B Cell Lymphoma 9 Protein; Bcl-9; Protein Legless Homolog) (FITC); anti-BCL9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2D4
• Specificity: Recognizes human BCL9.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 1426

• Applicable Applications for anti-BCL9 antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa1036-1135 from human BCL9 (NP_004317) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: DDDSPPARSPNLPSMNNMPGMGINTQNPRISGPNPVVPMPTLSPMGMTQPLSHSNQMPSPNAVGPNIPPHGVPMGPGLMSHNPIMGHGSQEPPMVPQGRM
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.74kD).)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to BCL9 on HeLa cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged BCL9 is ~0.03ng/ml as a capture antibody.)

Product Categories/Family for anti-BCL9 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 72256200
• NCBI GeneID: 607
• NCBI Accession #: NP_004317
• NCBI GenBank Nucleotide #: NM_004326
• UniProt Accession #: O00512
• NCBI Official Full Name: B-cell CLL/lymphoma 9 protein
• NCBI Official Synonym Full Names: BCL9 transcription coactivator
• NCBI Official Symbol: BCL9
• NCBI Official Synonym Symbols: LGS
• NCBI Protein Information: B-cell CLL/lymphoma 9 protein
• UniProt Protein Name: B-cell CLL/lymphoma 9 protein
• Protein Family: B-cell CLL/lymphoma 9 protein
• UniProt Gene Name: BCL9
• UniProt Entry Name: BCL9_HUMAN

NCBI Description

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Bcl-9: Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity. A chromosomal aberration involving BCL9 is found in a patient with precusor B-cell acute lymphoblastic leukemia (ALL). Translocation t(1;14)(q21;q32). This translocation leaves the coding region intact, but may have pathogenic effects due to alterations in the expression level of BCL9. Several cases of translocations within the 3'-UTR of BCL9 have been found in B-cell malignancies. Belongs to the BCL9 family.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; Golgi apparatus; cis-Golgi network

Molecular Function: protein binding; beta-catenin binding

Biological Process: regulation of transforming growth factor beta receptor signaling pathway; somatic stem cell maintenance; myotube differentiation involved in skeletal muscle regeneration; positive regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin