Mouse anti-Human BAX Monoclonal Antibody | anti-BAX antibody

BAX (Apoptosis Regulator BAX, Bcl-2-like Protein 4, Bcl2-L-4, BCL2L4) (FITC)

Cat. Num. AAA6146096

• Gene Names: BAX; BCL2L4
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BAX; Monoclonal Antibody; BAX (Apoptosis Regulator BAX; Bcl-2-like Protein 4; Bcl2-L-4; BCL2L4) (FITC); anti-BAX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 1F5-1B7
• Specificity: Recognizes human BAX.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 871

• Applicable Applications for anti-BAX antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-192 from human BAX (AAH14175) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTATIFVAGVLTASLTIWKKMG
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (46.86kD).)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to BAX on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between BCL2L1 and BAX. HeLa cells were stained with BCL2L1 rabbit purified polyclonal 1:1200 and BAX mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Product Categories/Family for anti-BAX antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 33991524
• NCBI GeneID: 581
• NCBI Official Full Name: Homo sapiens BCL2-associated X protein, mRNA
• NCBI Official Synonym Full Names: BCL2 associated X, apoptosis regulator
• NCBI Official Symbol: BAX
• NCBI Official Synonym Symbols: BCL2L4
• NCBI Protein Information: apoptosis regulator BAX
• Protein Family: Bax inhibitor

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Dec 2019]