Human Glycosaminoglycan xylosylkinase ELISA Kit | FAM20B elisa kit

Human Glycosaminoglycan xylosylkinase ELISA Kit

Cat. Num. AAA760189

• Gene Names: FAM20B; gxk1
• Reactivity: Human
• Synonyms: Glycosaminoglycan xylosylkinase; Human Glycosaminoglycan xylosylkinase ELISA Kit; FAM20B/Glycosaminoglycan xylosylkinase/Protein FAM20B/Xylose kinase/gxk1/XYLK; FAM20B elisa kit

• Reactivity: Human
• Sequence Length: 409

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FAM20B elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-FAM20B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FAM20B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FAM20B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FAM20B can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7662150
• NCBI GeneID: 9917
• NCBI Accession #: NP_055679.1
• NCBI GenBank Nucleotide #: NM_014864.3
• Molecular Weight: 46,432 Da
• NCBI Official Full Name: glycosaminoglycan xylosylkinase
• NCBI Official Synonym Full Names: family with sequence similarity 20 member B
• NCBI Official Symbol: FAM20B
• NCBI Official Synonym Symbols: gxk1
• NCBI Protein Information: glycosaminoglycan xylosylkinase
• UniProt Protein Name: Glycosaminoglycan xylosylkinase
• Protein Family: Glycosaminoglycan xylosylkinase
• UniProt Gene Name: FAM20B
• UniProt Entry Name: XYLK_HUMAN

Uniprot Description

FAM20B: Responsible for the 2-O-phosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature GAG chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG- protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1- O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-o-phosphorylation may influence the catalytic activity of B3GAT3 (GlcAT-I) which completes the precursor tetrasaccharide of GAG-protein linkage regions on which the repeating disaccharide region is synthesized. Belongs to the FAM20 family.

Protein type: EC 2.7.1.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; Golgi membrane; integral to membrane; nucleoplasm

Molecular Function: ATP binding; kinase activity; metal ion binding; phosphotransferase activity, alcohol group as acceptor

Biological Process: phosphorylation