Rabbit anti-Human, Mouse Dynamin-1-like Protein Polyclonal Antibody | anti-DNM1L antibody

Dynamin-1-like Protein (DNM1L, Dynamin Related Protein 1, DRP1, EC 3.6.5.5, Dynamin-like Protein, Dnm1p/Vps1p-like Protein, DVLP)

Cat. Num. AAA613855

• Gene Names: DNM1L; DLP1; DRP1; DVLP; VPS1; DYMPLE; HDYNIV; DYNIV-11; FLJ41912
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Dynamin-1-like Protein; Polyclonal Antibody; Dynamin-1-like Protein (DNM1L; Dynamin Related Protein 1; DRP1; EC 3.6.5.5; Dynamin-like Protein; Dnm1p/Vps1p-like Protein; DVLP); Anti -Dynamin-1-like Protein (DNM1L; anti-DNM1L antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Species Crossreactivity: This antibody reacts with the human and mouse protein. It has not been tested against any other species.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.

• Applicable Applications for anti-DNM1L antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.
• Immunogen: A synthetic peptide made to an N-terminal region within residues 1-100 of the human protein. [Swiss-Prot# O00429]. Homology: 100% Human, Monkey, and Hamster
• Cellular Localization: Cytoplasm; cytosol. Intracytoplasmic membrane; peripheral mambrane protein. Mainly cytosolic. Also membrane-associated. Localizes to mitochondia at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-DNM1L antibody

A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian cells. It plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses.

Product Categories/Family for anti-DNM1L antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 171460918
• NCBI GeneID: 10059
• NCBI Accession #: NP_005681.2
• NCBI GenBank Nucleotide #: NM_005690.3
• UniProt Accession #: O00429; O14541; O60709; Q7L6B3; Q8TBT7; Q9BWM1; Q9Y5J2; A8K4X9; B4DSU8; Q32Q67; Q59GN9
• Molecular Weight: 81,877 Da
• NCBI Official Full Name: dynamin-1-like protein isoform 3
• NCBI Official Synonym Full Names: dynamin 1-like
• NCBI Official Symbol: DNM1L
• NCBI Official Synonym Symbols: DLP1; DRP1; DVLP; VPS1; DYMPLE; HDYNIV; DYNIV-11; FLJ41912
• NCBI Protein Information: dynamin-1-like protein; dynamin-like protein 4; dynamin-like protein IV; Dnm1p/Vps1p-like protein; dynamin-related protein 1; dynamin family member proline-rich carboxyl-terminal domain less
• UniProt Protein Name: Dynamin-1-like protein
• Protein Family: Dynamin-1-like protein
• UniProt Gene Name: DNM1L
• UniProt Synonym Gene Names: DLP1; DRP1
• UniProt Entry Name: DNM1L_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated. [provided by RefSeq]

Uniprot Description

DRP1: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis- dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Can self-assemble in multimeric ring-like structures. Interacts with BCL2L1; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with FIS1. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in ite B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria. Interacts witn MID49 and MID51. Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. Belongs to the dynamin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Hydrolase; Endoplasmic reticulum; Mitochondrial; Motor; Microtubule-binding; EC 3.6.5.5

Chromosomal Location of Human Ortholog: 12p11.21

Cellular Component: Golgi apparatus; microtubule; protein complex; intracellular membrane-bound organelle; mitochondrion; synaptic vesicle membrane; coated pit; cytosol; mitochondrial outer membrane; membrane; perinuclear region of cytoplasm; cytoplasm; peroxisome; cell junction

Molecular Function: GTPase activity; identical protein binding; protein binding; protein homodimerization activity; GTP binding; ubiquitin protein ligase binding; lipid binding

Biological Process: peroxisome fission; mitochondrial fission; release of cytochrome c from mitochondria; positive regulation of protein secretion; positive regulation of apoptosis; metabolic process; apoptosis; regulation of protein oligomerization; endocytosis; mitochondrial fragmentation during apoptosis; protein homotetramerization; cell structure disassembly during apoptosis

Disease: Encephalopathy, Lethal, Due To Defective Mitochondrial And Peroxisomal Fission