Rabbit DRP1 Polyclonal Antibody | anti-DRP1 antibody

Rabbit polyclonal to Human DRP1

Cat. Num. AAA125938

• Gene Names: DNM1L; DLP1; DRP1; DVLP; EMPF; VPS1; DYMPLE; HDYNIV; DYNIV-11
• Reactivity: Human, mouse, rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: DRP1; Polyclonal Antibody; Rabbit polyclonal to Human DRP1; DRP1 Rabbit pab Antibody; anti-DRP1 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 699

• Applicable Applications for anti-DRP1 antibody: Western Blot (WB)
• Application Notes: WB: 1/500-1000
• Immunogen: A fusion protein of human DRP1
• Storage Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C or -80 degree C

Western Blot (WB)

(Western blot analysis of extracts of PC-12 cells, using DNM1L antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Related Product Information for anti-DRP1 antibody

Dynamin-related protein 1 (DRP1) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1). DRP1 affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5). The yeast ortholog of DRP1 clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3). The division of the mitochondria, which is required for apoptosis as well as normal cell growth and development, is controlled, in part, by the phosphorylation of DRP1 at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6). When phosphorylated at Ser616, DRP1 stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DRP1 is phosphorylated at Ser637 (reviewed in 6). Dephosphorylation of Ser637 by calcineurin reverses this inhibition (7). In addition to phosphorylation, sumoylation of DRP1 is also an enhancer of mitochondrial fission (8). Balancing fission and fusion events is essential for proper mitochondrial function. Mitochondrial defects are seen in a variety of neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, and Huntington's disease (reviewed in 6).

References

1. Praefcke, G.J. and McMahon, H.T. (2004) Nat Rev Mol Cell Biol 5, 133-47.
2. Taguchi, N. et al. (2007) J Biol Chem 282, 11521-9.
3. Smirnova, E. et al. (2001) Mol Biol Cell 12, 2245-56.
4. Smirnova, E. et al. (1998) J Cell Biol 143, 351-8.
5. Koch, A. et al. (2003) J Biol Chem 278, 8597-605.

NCBI and Uniprot Product Information

• NCBI GI #: 171460918
• NCBI GeneID: 10059
• NCBI Accession #: NP_005681.2
• NCBI GenBank Nucleotide #: NM_005690.3
• UniProt Accession #: O00429; O14541; O60709; Q7L6B3; Q8TBT7; Q9BWM1; Q9Y5J2; A8K4X9; B4DSU8
• Molecular Weight: 81,877 Da
• NCBI Official Full Name: dynamin-1-like protein isoform 3
• NCBI Official Synonym Full Names: dynamin 1-like
• NCBI Official Symbol: DNM1L
• NCBI Official Synonym Symbols: DLP1; DRP1; DVLP; EMPF; VPS1; DYMPLE; HDYNIV; DYNIV-11
• NCBI Protein Information: dynamin-1-like protein; dynamin-like protein 4; dynamin-like protein IV; Dnm1p/Vps1p-like protein; dynamin-related protein 1; dynamin family member proline-rich carboxyl-terminal domain less
• UniProt Protein Name: Dynamin-1-like protein
• UniProt Gene Name: DNM1L
• UniProt Synonym Gene Names: DLP1; DRP1; DVLP; Dymple; HdynIV
• UniProt Entry Name: DNM1L_HUMAN