Rabbit DNM1L Polyclonal Antibody | anti-DNM1L antibody

DNM1L Antibody

Cat. Num. AAA2516945

• Gene Names: DNM1L; DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antigen affinity purification
• Synonyms: DNM1L; Polyclonal Antibody; DNM1L Antibody; DLP1; DRP1; DVLP; DYMPLE; DYNIV-11; FLJ41912; HDYNIV; VPS1; anti-DNM1L antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 533

• Applicable Applications for anti-DNM1L antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Recombinant protein of human DNM1L
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-21804 / sc-32898
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using DNM1L antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophageal cancer using DNM1L antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using DNM1L antibody at dilution of 1:100 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using DNM1L antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-DNM1L antibody

Dynamin-related protein 1 (DNM1L) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1). DNM1L affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5). The yeast ortholog of DNM1L clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3). The division of the mitochondria, which is required for apoptosis, as well as normal cell growth and development is controlled, in part, by the phosphorylation of DNM1L at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6). When phosphorylated at Ser616, DNM1L stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DNM1L is phosphorylated at Ser637 (reviewed in 6). Dephosphorylation at Ser637 by calcineurin reverses this inhibition (7). In addition to phosphorylation, sumoylation of DNM1L is also an enhancer of mitochondrial fission (8). Balancing fission and fusion events is essential for proper mitochondrial function. Research studies have demonstrated mitochondrial defects in a variety of neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, and Huntington's disease (reviewed in 6).

NCBI and Uniprot Product Information

• NCBI GI #: 510937029
• NCBI GeneID: 10059
• NCBI Accession #: NP_001265395.1
• NCBI GenBank Nucleotide #: NM_001278466.1
• Molecular Weight: 82,057 Da
• NCBI Official Full Name: dynamin-1-like protein isoform 7
• NCBI Official Synonym Full Names: dynamin 1-like
• NCBI Official Symbol: DNM1L
• NCBI Official Synonym Symbols: DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV
• NCBI Protein Information: dynamin-1-like protein; Dnm1p/Vps1p-like protein; dynamin family member proline-rich carboxyl-terminal domain less; dynamin-like protein 4; dynamin-like protein IV; dynamin-related protein 1
• UniProt Protein Name: Dynamin-1-like protein
• Protein Family: Dynamin-1-like protein
• UniProt Gene Name: DNM1L
• UniProt Synonym Gene Names: DLP1; DRP1; DVLP; Dymple; HdynIV
• UniProt Entry Name: DNM1L_HUMAN

NCBI Description

This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]

Uniprot Description

DRP1: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis- dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Can self-assemble in multimeric ring-like structures. Interacts with BCL2L1; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with FIS1. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in ite B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria. Interacts witn MID49 and MID51. Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. Belongs to the dynamin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 3.6.5.5; Hydrolase; Apoptosis; Endoplasmic reticulum; Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 12p11.21

Cellular Component: Golgi apparatus; microtubule; mitochondrial outer membrane; protein complex; membrane; intracellular membrane-bound organelle; synaptic vesicle membrane; mitochondrion; perinuclear region of cytoplasm; cytoplasm; coated pit; peroxisome; cell junction; cytosol

Molecular Function: GTPase activity; identical protein binding; protein binding; protein homodimerization activity; GTP binding; ubiquitin protein ligase binding; lipid binding

Biological Process: peroxisome fission; mitochondrial fission; release of cytochrome c from mitochondria; positive regulation of protein secretion; apoptosis; metabolic process; positive regulation of apoptosis; regulation of protein oligomerization; endocytosis; mitochondrial fragmentation during apoptosis; protein homotetramerization; cell structure disassembly during apoptosis

Disease: Encephalopathy, Lethal, Due To Defective Mitochondrial And Peroxisomal Fission