Rabbit anti-Human, Rat Eukaryotic Translation Initiation Factor 2B, epsilon Polyclonal Antibody | anti-EIF2B5 antibody

Eukaryotic Translation Initiation Factor 2B, epsilon, phosphorylated (Ser539) (eIF2Be)

Cat. Num. AAA612917

• Gene Names: EIF2B5; CLE; CACH; LVWM; EIF-2B; EIF2Bepsilon
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Eukaryotic Translation Initiation Factor 2B; epsilon; Polyclonal Antibody; phosphorylated (Ser539) (eIF2Be); Anti -Eukaryotic Translation Initiation Factor 2B; anti-EIF2B5 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Specificity: Recognizes rat eIF2B when phosphorylated at Ser539. Species sequence homology: human (80%).
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

• Applicable Applications for anti-EIF2B5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000.
• Immunogen: Synthetic phosphopeptide derived from the region of rat eIF2Bepsilon that contains serine 535 (corresponding to serine 539 in the human sequence).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-EIF2B5 antibody

Eukaryotic Initiation Factor 2B (eIF2B) plays a key role in the initiation of mRNA translation in eukaryotic cells by acting as a guanine nucleotide exchange factor. The activity of eIF2B is regulated by a wide array of conditions. Stress inhibits its action, while stimulation of protein synthesis results in its activation. eIF2B, an 82kD subunit of eIF2B, is phosphorylated at serine 539 by glycogen synthase kinase-3 (GSK-3), which leads to inhibition of its guanine nucleotide exchange activity.

Product Categories/Family for anti-EIF2B5 antibody

Antibodies; Abs to Translation Initiation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 119598705
• NCBI GeneID: 8893
• UniProt Accession #: Q13144; Q541Z1; Q96D04; Q59HD8
• Molecular Weight: 80,380 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa, isoform CRA_b
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
• NCBI Official Symbol: EIF2B5
• NCBI Official Synonym Symbols: CLE; CACH; LVWM; EIF-2B; EIF2Bepsilon
• NCBI Protein Information: translation initiation factor eIF-2B subunit epsilon; OTTHUMP00000210774; OTTHUMP00000210847; eIF-2B GDP-GTP exchange factor subunit epsilon
• UniProt Protein Name: Translation initiation factor eIF-2B subunit epsilon
• Protein Family: Translation initiation factor
• UniProt Gene Name: EIF2B5
• UniProt Synonym Gene Names: EIF2BE
• UniProt Entry Name: EI2BE_HUMAN

NCBI Description

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq]

Uniprot Description

eIF2B-epsilon: eukaryotic translation initiation factor 2B, subunit 5. A translational regulatory protein that functions in the early steps of protein synthesis by catalyzing the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Mutation in EIF2B5 causes childhood ataxia with central nervous system hypomyelination/ vanishing white matter leukodystrophy.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: eukaryotic translation initiation factor 2B complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; translation initiation factor binding; guanyl-nucleotide exchange factor activity; translation initiation factor activity

Biological Process: myelination; response to peptide hormone stimulus; translation; astrocyte development; positive regulation of translational initiation; cellular protein metabolic process; astrocyte differentiation; ovarian follicle development; response to heat; response to glucose stimulus; translational initiation; negative regulation of translation initiation in response to stress; gene expression; oligodendrocyte development; positive regulation of GTPase activity

Disease: Leukoencephalopathy With Vanishing White Matter