Rabbit anti-Human, Mouse APLP2 Polyclonal Antibody | anti-APLP2 antibody

APLP2, CT (APLP2, APPL2, Amyloid-like protein 2, APPH, Amyloid protein homolog, CDEI box-binding protein) (PE)

Cat. Num. AAA6274378

• Gene Names: APLP2; APPH; APPL2; CDEBP; APLP-2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: APLP2; Polyclonal Antibody; CT (APLP2; APPL2; Amyloid-like protein 2; APPH; Amyloid protein homolog; CDEI box-binding protein) (PE); CDEI box-binding protein; anti-APLP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-APLP2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: APLP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-621 amino acids from the C-terminal region of human APLP2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(APLP2 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the APLP2 antibody detected the APLP2 protein (arrow).)

Western Blot (WB)

(APLP2 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the APLP2 antibody detected the APLP2 protein (arrow).)

Immunohistochemistry (IHC)

(APLP2 Antibody (C-term) (AP14673b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APLP2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-APLP2 antibody

APLP2 may play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein.

Product Categories/Family for anti-APLP2 antibody

Antibodies; Amyloid

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Tuli, A., et al. J. Biol. Chem. 284(49):34296-34307(2009). Kaden, D., et al. J. Cell. Sci. 122 (PT 3), 368-377 (2009): Tuli, A., et al. J. Immunol. 181(3):1978-1987(2008). Tuli, A., et al. Immunogenetics 60(6):303-313(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 214010181
• NCBI GeneID: 334
• NCBI Accession #: NP_001135748.1; NP_001633.1
• NCBI GenBank Nucleotide #: NM_001142276.1
• UniProt Accession #: Q06481; Q13861; Q14594; Q14662; Q71U10; Q7M4L3; Q9BT36
• Molecular Weight: 86,956 Da
• NCBI Official Full Name: amyloid-like protein 2 isoform 2
• NCBI Official Synonym Full Names: amyloid beta (A4) precursor-like protein 2
• NCBI Official Symbol: APLP2
• NCBI Official Synonym Symbols: APPH; APPL2; CDEBP; APLP-2
• NCBI Protein Information: amyloid-like protein 2; CDEI box-binding protein; amyloid precursor protein homolog HSD-2
• UniProt Protein Name: Amyloid-like protein 2
• Protein Family: Amyloid-like protein
• UniProt Gene Name: APLP2
• UniProt Synonym Gene Names: APPL2; APLP-2; CDEBP
• UniProt Entry Name: APLP2_HUMAN

NCBI Description

This gene encodes amyloid precursor- like protein 2 (APLP2), which is a member of the APP (amyloid precursor protein) family including APP, APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

APLP2: May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein. Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts. Belongs to the APP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Membrane protein, integral; Cell surface; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q24

Cellular Component: membrane; integral to membrane; plasma membrane; nucleus

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; identical protein binding; protein binding; DNA binding; transition metal ion binding

Biological Process: cholesterol metabolic process; G-protein coupled receptor protein signaling pathway; extracellular matrix organization and biogenesis; cellular copper ion homeostasis; suckling behavior; regulation of protein binding; forebrain development; midbrain development; regulation of epidermal growth factor receptor activity; mating behavior; locomotory behavior; neuromuscular process controlling balance