Rabbit anti-Human, Mouse RSK2 Polyclonal Antibody | anti-RPS6KA3 antibody

RSK2, NT (Ribosomal S6 Kinase 2, RSK-2, p90-RSK2, pp90RSK2, 90kD Ribosomal Protein S6 Kinase 3, p90-RSK 3, p90RSK3, Insulin-stimulated Protein Kinase 1, ISPK1, ISPK-1, MAP Kinase-activated Protein Kinase 1b, MAPK-activated Protein Kinase 1b, MAPKAP Kinase

Cat. Num. AAA612359

• Gene Names: RPS6KA3; CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RSK2; Polyclonal Antibody; NT (Ribosomal S6 Kinase 2; RSK-2; p90-RSK2; pp90RSK2; 90kD Ribosomal Protein S6 Kinase 3; p90-RSK 3; p90RSK3; Insulin-stimulated Protein Kinase 1; ISPK1; ISPK-1; MAP Kinase-activated Protein Kinase 1b; MAPK-activated Protein Kinase 1b; MAPKAP Kinase; Anti -RSK2; anti-RPS6KA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RSK2 at ~84kD. Species crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-RPS6KA3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-3ug/ml. Identifies a single band at ~84 kD
• Immunogen: Synthetic peptide corresponding to the N-terminal region of the human and mouse RSK2 (ribosomal S6 kinase 2, ribosomal protein S6 kinase alpha 3, 90kD ribosomal protein S6 kinase 3, and MAP kinase-activated protein kinase 1b).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-RPS6KA3 antibody

Members of the RSK (the p90 ribosomal S6 kinase) family of serine/threonine kinases are substrates of ERK (the Ras/extracellular signal-regulated kinase) (1). The RSK family consists of four isoforms (RSK1 to -4) and two structurally related cousins, called RSK-like protein kinase (RLPK/MSK1) and RSKB (MSK2). RSK2 is expressed in most tissues including heart, brain, placenta, liver, kidney and pancreas, with predominant expression in skeletal muscle (4-5). The small death effector domain protein PEA-15 inhibits RSK2 nuclear translocation, but the biological function of this interaction is unknown6. Activated RSK2 can also be found in the cytoplasm of stimulated cells suggesting that RSK2 substrates may exist in both nuclear and cytoplasmic compartments (7). A phosphate-binding pocket in the kinase domain of RSK2 is used for intramolecular interaction with its own phosphorylated hydrophobic motif, an interaction that induces a synergistic stimulation of RSK2 catalytic activity, whereas mutation of the phosphate-binding pocket leads to a reduction in the overall kinase activity of RSK28. Defects in the RSK2 gene are the cause of Coffin-Lowry syndrome, an X-linked dominant disorder characterized by psychomotor and growth retardation and facial, hand and skeletal malformations9-10. Numerous mutations have been identified in the RSK2 gene that result in truncated or inactive RSK2 proteins (11-12).

Product Categories/Family for anti-RPS6KA3 antibody

Antibodies; Abs to Protein Kinases; Ribosomal

NCBI and Uniprot Product Information

• NCBI GI #: 4759050
• NCBI GeneID: 6197
• NCBI Accession #: NP_004577.1
• NCBI GenBank Nucleotide #: NM_004586.2
• UniProt Accession #: P51812; Q4VAP3; Q59H26; Q5JPK8; Q7Z3Z7; B2R9V4
• Molecular Weight: 83,736 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-3
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 90kDa, polypeptide 3
• NCBI Official Symbol: RPS6KA3
• NCBI Official Synonym Symbols: CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
• NCBI Protein Information: ribosomal protein S6 kinase alpha-3; RSK-2; p90-RSK 3; MAPKAPK-1b; S6K-alpha-3; MAPKAP kinase 1b; ribosomal S6 kinase 2; MAPK-activated protein kinase 1b; insulin-stimulated protein kinase 1; MAP kinase-activated protein kinase 1b
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-3
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KA3
• UniProt Synonym Gene Names: ISPK1; MAPKAPK1B; RSK2; S6K-alpha-3; p90-RSK 3; p90RSK3; ISPK-1; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2
• UniProt Entry Name: KS6A3_HUMAN

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]

Uniprot Description

RSK2: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: Xp22.2-p22.1

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; caspase inhibitor activity; magnesium ion binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: axon guidance; central nervous system development; nerve growth factor receptor signaling pathway; stress-activated MAPK cascade; response to lipopolysaccharide; negative regulation of caspase activity; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; synaptic transmission; skeletal development; toll-like receptor 4 signaling pathway; regulation of transcription in response to stress; MyD88-independent toll-like receptor signaling pathway; regulation of translation in response to stress; cell cycle; positive regulation of cell growth; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; positive regulation of cell differentiation; negative regulation of apoptosis

Disease: Mental Retardation, X-linked 19; Coffin-lowry Syndrome