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Rabbit Guanine Nucleotide Dissociation Inhibitor Polyclonal Antibody | anti-PAS_chr1-4_0063 antibody

Guanine Nucleotide Dissociation Inhibitor (GDI)

Reactivity
Bovine, Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
Guanine Nucleotide Dissociation Inhibitor; Polyclonal Antibody; Guanine Nucleotide Dissociation Inhibitor (GDI); Anti -Guanine Nucleotide Dissociation Inhibitor (GDI); anti-PAS_chr1-4_0063 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Speci c for GDI-1 (~50kD). GDI-1 is also referred to as GDI-a and Rab3A GDI (1). Crossreactivity to GDI-2 (~46kD) has been observed. A synthetic peptide, corresponding to a 20 amino acid sequence of bovine brain Rab Guanine Nucleotide Dissociation Inhibitor (GDI), was conjugated to carrier protein and used as the immunogen. This sequence is conserved in both mouse and rat GDI proteins. Human, mouse and rat. Reactivity has been con rmed by Western blot analysis of extracts derived from human HEK293 cells and of recombinant bovine Rab-GDI.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Applicable Applications for anti-PAS_chr1-4_0063 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in ELISA, Western Blotting, Immunoprecipitation.
Dilution: ELISA: 0.5-1ug/ml
Immunoprecipitation: 5-10ug
Western Blotting (2) : 1ug/ml
Immunogen
A 20 amino acid synthetic peptide derived from bovine brain Rab Guanine Nucleotide Dissociation Inhibitor (GDI).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-PAS_chr1-4_0063 antibody
Small molecular weight GTP-binding proteins of the Rab family have been shown to play an essential role in the regulation of vesicular trafficking. The cycling of these proteins between the inactive (GDP-bound) and active (GTP-bound) forms is thought to catalyze docking to specific target membranes. To date, several accessory proteins which serve to regulate the association of Rab proteins with GTP or GDP have been identified(4,5,6). The best characterized of these Rab accessory proteins is Rab3A GDI(4,5,7,8). Rab3A GDI forms cytosolic complexes with Rab3A and, in vitro, promotes the dissociation of Rab3A from membranes(7,8). Two other isoforms of GDI have recently been isolated and have been termed GDI b and GDI-2, respectively(3,5). Studies have revealed that the proportion of Rab proteins found associated with a particular GDI isoform depends upon the abundance of the specific GDI in the cytosol, as well as on the tissue and cell type examined(5,6,7).
Product Categories/Family for anti-PAS_chr1-4_0063 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p
NCBI Official Symbol
PAS_chr1-4_0063
NCBI Protein Information
G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p

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Product Notes

The PAS_chr1-4_0063 (Catalog #AAA611886) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Guanine Nucleotide Dissociation Inhibitor (GDI) reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Guanine Nucleotide Dissociation Inhibitor can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Dilution: ELISA: 0.5-1ug/ml Immunoprecipitation: 5-10ug Western Blotting (2) : 1ug/ml. Researchers should empirically determine the suitability of the PAS_chr1-4_0063 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Guanine Nucleotide Dissociation Inhibitor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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