Goat anti-Mouse Paired Immunoglobulin-Like Type 2 Receptor-Ligand Polyclonal Antibody | anti-Cd99 antibody
Paired Immunoglobulin-Like Type 2 Receptor-Ligand (PILR-L, CD99, MIC2)
Gene Names
Cd99; D4; Pilr-l; pilr-1; 1110061M03Rik; 2410026K10Rik
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by mouse PILR-L affinity chromatography.
Synonyms
Paired Immunoglobulin-Like Type 2 Receptor-Ligand; Polyclonal Antibody; Paired Immunoglobulin-Like Type 2 Receptor-Ligand (PILR-L; CD99; MIC2); Anti -Paired Immunoglobulin-Like Type 2 Receptor-Ligand (PILR-L; anti-Cd99 antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Selected for its ability to recognize mouse PILR-L in direct ELISAs and Western Blots.
Purity/Purification
Affinity Purified
Purified by mouse PILR-L affinity chromatography.
Purified by mouse PILR-L affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Applicable Applications for anti-Cd99 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse PILR-L. The detection limit for rmPILR-L is approximately 2 ng/lane under non-reducing and reducing conditions, respectively., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse PILR-L. The detection limit for rmPILR-L is approximately 0.1 ng/well. In this format, this antibody shows less than 1% crossreactivity with rmPILR- and rmPILR-., Optimal dilutions should be determined by each laboratory for each application.
Immunogen
NS0-derived rmPILR-L (aa25-138)
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Cd99 antibody
PILR-L (also known as CD99 and MIC2) is a 125 amino acid, 24-30 kD glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. PILR-L has been implicated in processes like homotypic cell adhesion, diapedesis and oncosupression. The extracellular domain of the mouse PILR-L protein used as immunogen is 45% identical to the human counterpart.
Product Categories/Family for anti-Cd99 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,783 Da
NCBI Official Full Name
paired immunoglobin-like type 2 receptor-ligand
NCBI Official Synonym Full Names
CD99 antigen
NCBI Official Symbol
Cd99
NCBI Official Synonym Symbols
D4; Pilr-l; pilr-1; 1110061M03Rik; 2410026K10Rik
NCBI Protein Information
CD99 antigen; paired immunoglobin-like type 2 receptor-ligand
UniProt Protein Name
CD99 antigen
Protein Family
UniProt Gene Name
Cd99
UniProt Synonym Gene Names
Pilrl
UniProt Entry Name
CD99_MOUSE
Uniprot Description
CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane
Molecular Function: protein binding
Biological Process: myeloid dendritic cell activation
Research Articles on Cd99
Similar Products
Product Notes
The Cd99 cd99 (Catalog #AAA611785) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Paired Immunoglobulin-Like Type 2 Receptor-Ligand (PILR-L, CD99, MIC2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Paired Immunoglobulin-Like Type 2 Receptor-Ligand can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse PILR-L. The detection limit for rmPILR-L is approximately 2 ng/lane under non-reducing and reducing conditions, respectively., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse PILR-L. The detection limit for rmPILR-L is approximately 0.1 ng/well. In this format, this antibody shows less than 1% crossreactivity with rmPILR- and rmPILR-., Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the Cd99 cd99 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Paired Immunoglobulin-Like Type 2 Receptor-Ligand, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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