Rabbit anti-Human MLK4 Polyclonal Antibody | anti-MLK4 antibody

MLK4, NT (Mixed Lineage Kinase 4, dJ862P8.3, KIAA1804, Mitogen-activated Protein Kinase Kinase Kinase, RP5-862P8.2) (Biotin)

Cat. Num. AAA6488379

• Gene Names: KIAA1804; MLK4; dJ862P8.3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MLK4; Polyclonal Antibody; NT (Mixed Lineage Kinase 4; dJ862P8.3; KIAA1804; Mitogen-activated Protein Kinase Kinase Kinase; RP5-862P8.2) (Biotin); EC=2.7.11.25; anti-MLK4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MLK4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MLK4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human MLK4 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MLK4 (arrow) using rabbit polyclonal MLK4 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KIAA1804 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-MLK4 antibody

Antibodies; Protein Kinases

References

1. Gregory, S.G., Nature 441(7091), 315-321 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 155723000
• NCBI GeneID: 84451
• NCBI Accession #: NP_115811
• NCBI GenBank Nucleotide #: NM_032435
• UniProt Accession #: Q5TCX8; Q5TCX7; Q5TCX9; Q8WWN1; Q8WWN2; Q96JM1; B2RN34
• Molecular Weight: 52,817 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase MLK4
• NCBI Official Symbol: KIAA1804
• NCBI Official Synonym Symbols: MLK4; dJ862P8.3
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase MLK4
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase MLK4
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MLK4
• UniProt Synonym Gene Names: KIAA1804
• UniProt Entry Name: M3KL4_HUMAN

Uniprot Description

MLK4: Activates the JUN N-terminal pathway. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Protein kinase, TKL; Kinase, protein; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; MLK subfamily

Chromosomal Location of Human Ortholog: 1q42

Molecular Function: protein homodimerization activity; MAP kinase kinase kinase activity; protein-tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; activation of MAPKK activity; protein amino acid autophosphorylation; protein amino acid phosphorylation; activation of JNK activity