anti-Human CARD4 Polyclonal Antibody | anti-CARD4 antibody

Anti - CARD4 (Caspase Recrutiment Domain 4, Nod 1)

Cat. Num. AAA611696

• Gene Names: NOD1; CARD4; NLRC1; CLR7.1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Serum
• Synonyms: CARD4; Polyclonal Antibody; Anti - CARD4 (Caspase Recrutiment Domain 4; Nod 1); CARD4 (Caspase Recrutiment Domain 4; anti-CARD4 antibody

• Host: Host: Rabbit; Source: Human
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Sequence is 89% conserved in mouse NOD1. It has no significant homology with ced-3/4, Apaf-1, NOD-2 or other proteins.
• Purity/Purification: Serum
• Form/Format: Serum; Supplied as a liquid, neat serum, 40% glycerol.
• Sequence Length: 953

• Applicable Applications for anti-CARD4 antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA, Western Blotting. Other applications not tested.; Dilution: Western Blotting Antiserum at 1:1K-1:3K; affinity purified at 1-5ug/ml using ECL.; ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.; Optimal dilutions to be determined by the researcher.
• Immunogen: 19-aa peptide from human Nod1
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CARD4 antibody

Apoptosis or programmed cell death is a fundamental cellular process that is essential for normal tissue development and abnormal growth. Apoptosis is driven by two classes of specialized proteases known as caspases (Cysteine Aspartase). Several key factors are released from the mitochondria that regulate apoptosis. The first such factor (Cytochrome-C) to be described binds to a cytoplasmic scaffolding protein called Apaf-1 (Apoptosis Protease activating factor-1), a homolog of C. elegans Ced-4. Both Apaf-1 and Ced-4 are composed of an N-terminal Caspase Recrutiment domain (CARD) linked to a Nuceoltide-bidning domain (NBD), also known as NB-ARC or NOD domain. Ced-4 and Apaf-1 self-associate via the NBD and activate Casp-3 and -9. In response to certain apoptotic stimuli, Cytochrome-C is released from the mitochondria and binds to Apaf-1 to form a ternary complex with, and activate, the initiator pro-caspase-9. Active caspase-9 then turns on downstream effector caspases, initiating apoptosis. Recently, Additional members of Apaf-1 family, NOD1/CARD4 and NOD2 have been cloned and characterized.

Product Categories/Family for anti-CARD4 antibody

Abs to Apoptosis

References

• 1. Inohara N et al (1999) JBC 274, 14560-14567; Bertin J et al (1999) JBC 274, 12955-12953; Ogura Y et al (2001) JBC 276, 4812-4818; Cecconi, F. et al (1998) Cell 94, 727-737; Zou et al (1997) Cell 90, 405-413; Soengas MS et al (2001) Nature 409, 207-211; Srinivasulam SM et al (1998) Mol. Cell 1, 949-957.

NCBI and Uniprot Product Information

• NCBI GI #: 4760400
• NCBI GeneID: 10392
• UniProt Accession #: Q9Y239; Q549U4; Q8IWF5; Q7Z2K1
• Molecular Weight: 107,691 Da
• NCBI Official Full Name: CARD4
• NCBI Official Synonym Full Names: nucleotide-binding oligomerization domain containing 1
• NCBI Official Symbol: NOD1
• NCBI Official Synonym Symbols: CARD4; NLRC1; CLR7.1
• NCBI Protein Information: nucleotide-binding oligomerization domain-containing protein 1; OTTHUMP00000158742; NLR family, CARD domain containing 1; caspase recruitment domain family, member 4; caspase recruitment domain-containing protein 4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1
• UniProt Protein Name: Nucleotide-binding oligomerization domain-containing protein 1
• UniProt Gene Name: NOD1
• UniProt Synonym Gene Names: CARD4
• UniProt Entry Name: NOD1_HUMAN