Human nucleotide-binding oligomerization domain containing 1 ELISA Kit | NOD1 elisa kit

Human Nucleotide-binding oligomerization domain-containing protein 1, NOD1 ELISA Kit

Cat. Num. AAA916006

• Gene Names: NOD1; CARD4; NLRC1; CLR7.1
• Reactivity: Human
• Synonyms: nucleotide-binding oligomerization domain containing 1; Human Nucleotide-binding oligomerization domain-containing protein 1; NOD1 ELISA Kit; Human Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) ELISA kit; CARD4; CLR7.1; NLRC1; NLR family; CARD domain containing 1; caspase recruitment domain family; member 4; caspase recruitment domain-containing protein 4; nucleotide-binding oligomerization domain; leuc; NOD1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human NOD1. No significant cross-reactivity or interference between human NOD1 and analogues was observed.
• Sequence Length: 953

• Samples: Serum, plasma, tissue homogenates, cell lysates.
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml -1000 pg/ml.
• Sensitivity: 3.9 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOD1 elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NOD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5174617
• NCBI GeneID: 10392
• NCBI Accession #: NP_006083.1
• NCBI GenBank Nucleotide #: NM_006092.2
• UniProt Accession #: Q9Y239; Q549U4; Q8IWF5; B4DTU3
• Molecular Weight: 107,691 Da
• NCBI Official Full Name: nucleotide-binding oligomerization domain-containing protein 1
• NCBI Official Synonym Full Names: nucleotide-binding oligomerization domain containing 1
• NCBI Official Symbol: NOD1
• NCBI Official Synonym Symbols: CARD4; NLRC1; CLR7.1
• NCBI Protein Information: nucleotide-binding oligomerization domain-containing protein 1; NLR family, CARD domain containing 1; caspase recruitment domain family, member 4; caspase recruitment domain-containing protein 4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1
• UniProt Protein Name: Nucleotide-binding oligomerization domain-containing protein 1
• Protein Family: Nucleotide-binding oligomerization domain-containing protein
• UniProt Gene Name: NOD1
• UniProt Synonym Gene Names: CARD4
• UniProt Entry Name: NOD1_HUMAN

NCBI Description

This gene encodes a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid. Multiple alternatively spliced transcript variants differring in the 5' UTR have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Oct 2009]

Uniprot Description

NOD1: Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2. Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: basolateral plasma membrane; apical plasma membrane; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; peptidoglycan binding; protein homodimerization activity; CARD domain binding; caspase activator activity; ATP binding

Biological Process: detection of bacterium; activation of MAPK activity; apoptosis; positive regulation of dendritic cell antigen processing and presentation; stress-activated MAPK cascade; positive regulation of caspase activity; positive regulation of JNK cascade; defense response; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; interleukin-8 biosynthetic process; JNK cascade; inflammatory response; detection of biotic stimulus; toll-like receptor 4 signaling pathway; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; protein oligomerization; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway