TIMP-1 recombinant protein

Recombinant mouse TIMP-1 protein

Cat. Num. AAA201353

• Gene Names: Timp1; EPA; Clgi; Timp; TIMP-1; TPA-S1
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: TIMP-1; Recombinant mouse TIMP-1 protein; Timp1; Cl; Clgi; EPA; Timp; TPA-S1; Metalloproteinase inhibitor 1; Collagenase inhibitor 16C8 fibroblast; Erythroid-potentiating activity; TPA-induced protein; TIMP-1 recombinant protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) 10% glycerol
• Concentration: 0.5mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 25-205aa
• Sequence: CSCAPPHPQT AFCNSDLVIR AKFMGSPEIN ETTLYQRYKI KMTKMLKGFK AVGNAADIRY AYTPVMESLC GYAHKSQNRS EEFLITGRLR NGNLHISACS FLVPWRTLSP AQQRAFSKTY SAGCGVCTVF PCLSIPCKLE SDTHCLWTDQ VLVGSEDYQS RHFACLPRNP GLCTWRSLGA R

• Applicable Applications for TIMP-1 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Mouse
• Tag: His-Tag
• Predicted N-term: Cys25
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for TIMP-1 recombinant protein

TIMP-1, also known as metalloproteinase inhibitor 1, is a member of the TIMP family. Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. It has been identified as a multifunctional molecule with divergent functions. Recombinant Mouse TIMP-1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for TIMP-1 recombinant protein

Angiogenesis; Cardiovascular; Adhesion & Movement; Proteins

References

M Toricelli, et al. (2013) Molecular Cancer 12:1095. Gardner., et al. (2003) J. Neurosci. Res. 74:801-806. Osthues A., et al. (1992) FEBS. Lett. 296:16-20.

NCBI and Uniprot Product Information

• NCBI GI #: 113205065
• NCBI GeneID: 21857
• NCBI Accession #: NP_035723.2
• NCBI GenBank Nucleotide #: NM_011593.2
• UniProt Accession #: P12032; P20064; Q61720
• Molecular Weight: 22,628 Da
• NCBI Official Full Name: metalloproteinase inhibitor 1 isoform a
• NCBI Official Synonym Full Names: tissue inhibitor of metalloproteinase 1
• NCBI Official Symbol: Timp1
• NCBI Official Synonym Symbols: EPA; Clgi; Timp; TIMP-1; TPA-S1
• NCBI Protein Information: metalloproteinase inhibitor 1
• UniProt Protein Name: Metalloproteinase inhibitor 1
• UniProt Gene Name: Timp1
• UniProt Synonym Gene Names: Timp; Timp-1; EPA; TIMP-1
• UniProt Entry Name: TIMP1_MOUSE

Uniprot Description

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: basement membrane; extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: cytokine activity; metalloendopeptidase inhibitor activity; protease binding

Biological Process: cell activation; negative regulation of apoptosis; negative regulation of catalytic activity; negative regulation of membrane protein ectodomain proteolysis; positive regulation of cell proliferation; response to cytokine stimulus; response to hormone stimulus