Rabbit anti-Human Vav3 Polyclonal Antibody

Vav3 (vav 3 oncogene, Oncogene VAV23 Guanine nucleotide exchange factor, Gef, GDP/GTP Exchange Factor, Gef)

Cat. Num. AAA610795

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Vav3; Polyclonal Antibody; Vav3 (vav 3 oncogene; Oncogene VAV23 Guanine nucleotide exchange factor; Gef; GDP/GTP Exchange Factor; Gef); Anti -Vav3 (vav 3 oncogene; anti-Vav3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Vav3, Mr ~98kD. Additional non-specific bands detected in long exposures at Mr ~38-40kD. A non-specific band at Mr ~95kD was detected in Jurkat lysates.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.

• Applicable Applications for anti-Vav3 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.; Dilution: Western Blot: 0.5-2ug/ml detected Vav3 in RIPA lysates from HeLa and Jurkat cells.; Immunoprecipitation: Reported to immunoprecipitate Vav3 from Jurkat cell lysate and SU86.86 pancreatic tumor cell lysate.; Immunocytochemistry: Reported to detect Vav3 in Jurkat and SU86.86 pancreatic tumor cells.; Immunohistochemistry: Reported to detect Vav3 in pancreatic adenocarcinoma sections.
• Immunogen: Synthetic peptide corresponding to aa566-591 (NSGEQGTLKLPEKRTNGLRRTPKQVD) of human Vav3 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS610795 (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Vav3 (98kD).)

Related Product Information for anti-Vav3 antibody

Vav proteins belong to the guanidine nucleotide exchange factor family of proteins that couple receptors to Rho-GTPases. The VAV family has currently three members in mammalian cells (VAV 1, VAV 2, and VAV 3). VAV 1 contains multiple protein binding motifs, indicating a role for VAV 1 as an adaptor protein linking cell surface receptors to downstream signaling proteins. VAV 1 is exclusively expressed in hematopoietic cells and plays a significant role in lymphocyte development and antigen receptor mediated activation of NFAT and NF(kappa)B, and in more delayed responses such as the production of cytokines, including IL-2, IL-3, IFN lambda, and TNF alpha. VAV 3 is also involved in activating JNK, p21-activated kinase (PAK) and PLC lambda, implicating a critical role in cytoskeletal rearrangement. VAV 3 is inducibly tyrosine phosphorylated in response to stimulation of the epidermal growth factor (EGFR), platelet-derived growth factor (PDGFR) and T-cell receptors (TCR), enhancing its guanine nucleotide exchange factor activity in vitro. Tyrosine 173 is the regulatory phosphorylation site of VAV 3.

Product Categories/Family for anti-Vav3 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 117616734
• NCBI Official Full Name: Vav3
• Protein Family: Guanine nucleotide exchange factor