Rabbit anti-Human Vav3 Polyclonal Antibody | anti-Vav3 antibody

Vav3 (vav 3 oncogene, Oncogene VAV23 Guanine nucleotide exchange factor, Gef, GDP/GTP Exchange Factor, Gef) (MaxLight 750)

Cat. Num. AAA6505671

• Reactivity: Human
• Applications: Immunocytochemistry, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Vav3; Polyclonal Antibody; Vav3 (vav 3 oncogene; Oncogene VAV23 Guanine nucleotide exchange factor; Gef; GDP/GTP Exchange Factor; Gef) (MaxLight 750); anti-Vav3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Vav3, Mr ~98kD. Additional non-specific bands detected in long exposures at Mr ~38-40kD. A non-specific band at Mr ~95kD was detected in Jurkat lysates.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-Vav3 antibody: Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: ICC: Reported to detect Vav3 in Jurkat and SU86.86 pancreatic tumor cells.; WB: 0.5-2ug/ml detected Vav3 in RIPA lysates from HeLa and Jurkat cells.; IHC: Reported to detect Vav3 in pancreatic adenocarcinoma sections.; IP: Reported to immunoprecipitate Vav3 from Jurkat cell lysate and SU86.86 pancreatic tumor cell lysate.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to aa566-591 (NSGEQGTLKLPEKRTNGLRRTPKQVD) of human Vav3 (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS610795 (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Vav3 (98kD).)

Product Categories/Family for anti-Vav3 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Lee, H. J. et al., (2012) Ann. Surgical Oncology DOI: 10.1245/s10434-012-2553-6. 1. Cao, Y., et.al., EMBO J. 21: 4809-4819, 2002. 2. Turner, M. and D.D. Billadeau, Nature Rev. 2: 476-486, 2002.

NCBI and Uniprot Product Information

• NCBI GI #: 120432042
• NCBI GeneID: 10451
• NCBI Accession #: NP_001073343.1
• NCBI GenBank Nucleotide #: NM_006113
• UniProt Accession #: Q9UKW4; O60498; O95230; Q9Y5X8; B1AMM0; B1APV5; B4E232; B7ZLR1; E9PQ97
• Molecular Weight: 97,776 Da
• NCBI Official Full Name: guanine nucleotide exchange factor VAV3 isoform 2
• NCBI Official Synonym Full Names: vav 3 guanine nucleotide exchange factor
• NCBI Official Symbol: VAV3
• NCBI Protein Information: guanine nucleotide exchange factor VAV3; VAV-3; vav 3 oncogene
• UniProt Protein Name: Guanine nucleotide exchange factor VAV3
• Protein Family: Guanine nucleotide exchange factor
• UniProt Gene Name: VAV3
• UniProt Synonym Gene Names: VAV-3
• UniProt Entry Name: VAV3_HUMAN

NCBI Description

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

VAV3: a guanine nucleotide exchange factor (GEF) that activates RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by activated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion. Interacts with the PH domain of APS. Interacts (via SH2 domains) with the phosphorylated form of EPHA2. Interacts with ROS1; constitutive interaction that mediates VAV3 phosphorylation. Down-regulated by EGF and TGF-beta. Four isoforms of the human protein are produced by alternative promoter. Isoform 1 and isoform 3 are widely expressed; both are expressed at very low levels in skeletal muscle. In keratinocytes, isoform 1 is less abundant than isoform 3. Isoform 3 is detected at very low levels, if any, in adrenal gland, bone marrow, spleen, fetal brain and spinal chord; in these tissues, isoform 1 is readily detectable.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Adaptor/scaffold; Actin-binding; GEFs, Rac/Rho; GEFs

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: plasma membrane; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; metal ion binding; Rac guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; GTPase activator activity; epidermal growth factor receptor binding

Biological Process: response to drug; lamellipodium biogenesis; integrin-mediated signaling pathway; platelet activation; neutrophil chemotaxis; axon guidance; positive regulation of cell adhesion; positive regulation of signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of phosphoinositide 3-kinase activity; vesicle fusion; regulation of small GTPase mediated signal transduction; B cell receptor signaling pathway; small GTPase mediated signal transduction; ephrin receptor signaling pathway; positive regulation of B cell proliferation; innate immune response; angiogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; response to DNA damage stimulus