Western Blot (WB)
(Western Blot of mouse kidney cytoplasmic extracts using MBS614066 (1:10,000).)
Rabbit Nedd4, WW2 domain Polyclonal Antibody | anti-Nedd4 antibody
Nedd4, WW2 domain
Gene Names
Nedd4; CG32184; CG42279; CG7555; DmelCG42279; dNedd4; DNedd4; dNedd4-1; nedd4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Nedd4; WW2 domain; Polyclonal Antibody; Anti -Nedd4; anti-Nedd4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat Nedd4, Mr ~115kD. Degradation products were also detected at Mr 55 and 60kD. Species Crossreactivity: mouse and human.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Applicable Applications for anti-Nedd4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:10,000 detects Nedd4, WW2 domain on 10ug of mouse kidney cytosol. Mouse kidney cytosol lysate was resolved by electrophoresis, transferred to PVDF and probed with N0577-15. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunohistochemistry: Reported to detect Nedd4 in mouse kidney, lung and distal colon sections.
Dilution: Western Blot: 1:10,000 detects Nedd4, WW2 domain on 10ug of mouse kidney cytosol. Mouse kidney cytosol lysate was resolved by electrophoresis, transferred to PVDF and probed with N0577-15. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunohistochemistry: Reported to detect Nedd4 in mouse kidney, lung and distal colon sections.
Immunogen
GST fusion protein corresponding to aa395-462 of the WW2 domain of rat Nedd4 (NM_006154).
Positive Control
Kidney cytoplasmic extracts
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot of mouse kidney cytoplasmic extracts using MBS614066 (1:10,000).)
Western Blot (WB)
(Western Blot of mouse kidney cytoplasmic extracts using MBS614066 (1:10,000).)
Related Product Information for anti-Nedd4 antibody
Neuronal precursor cell-expressed developmentally downregulated 4 (Nedd4) is an ~120kD E3 ubiquitin ligase. Nedd4 is involved in regulation of ubitiquin-targeted degradation of proteins. Nedd4 interacts with many different E2 enzymes, including human Ubc4, UbcH5B, UbcH5C, UbcH6 and UbcH7, suggesting it plays a role in multiple E2 ubiquitination pathways. Nedd4 contains a C2 domain and several WW domains, which recognize membrane surfaces and Pro-Tyr motifs, respectively. Nedd4 polyubiquitinates the epithelial sodium channel, and mutations that disrupt association between Nedd4 and the channel lead to Liddle's syndrome, a hyperaldosteronism-like, hypertension disease.
Product Categories/Family for anti-Nedd4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Nedd4, isoform H
NCBI Official Symbol
Nedd4
NCBI Official Synonym Symbols
CG32184; CG42279; CG7555; DmelCG42279; dNedd4; DNedd4; dNedd4-1; nedd4
NCBI Protein Information
CG42279 gene product from transcript CG42279-RJ; Nedd4, isoform J; CG42279-PJ; Nedd4-PJ; DNedd4; Nedd4, isoform I; CG42279-PI; Nedd4-PI; Nedd4, isoform G; CG42279-PG; Nedd4-PG; Nedd4, isoform K; CG42279-PK; Nedd4-PK; Nedd4, isoform H; CG42279-PH; Nedd4-PH; Nedd4, isoform F; CG42279-PF; Nedd4-PF; Nedd4, isoform E; CG42279-PE; Nedd4-PE
Protein Family
Research Articles on Nedd4
Similar Products
Product Notes
The Nedd4 (Catalog #AAA614066) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nedd4, WW2 domain reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nedd4, WW2 domain can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:10,000 detects Nedd4, WW2 domain on 10ug of mouse kidney cytosol. Mouse kidney cytosol lysate was resolved by electrophoresis, transferred to PVDF and probed with N0577-15. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunohistochemistry: Reported to detect Nedd4 in mouse kidney, lung and distal colon sections. Researchers should empirically determine the suitability of the Nedd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nedd4, WW2 domain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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