Mouse anti-Human ADAM8 Ectodomain Monoclonal Antibody | anti-ADAM8 antibody

ADAM8 Ectodomain (a Disintegrin and Metalloproteinase Domain 8, MS2, CD156, CD156 MS2)

Cat. Num. AAA608666

• Gene Names: ADAM8; MS2; CD156; MGC134985
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Purified by Protein G affinity chromatography. ; Endotoxin: <0.1EU/ug (LAL)
• Synonyms: ADAM8 Ectodomain; Monoclonal Antibody; ADAM8 Ectodomain (a Disintegrin and Metalloproteinase Domain 8; MS2; CD156; CD156 MS2); Anti -ADAM8 Ectodomain (a Disintegrin and Metalloproteinase Domain 8; anti-ADAM8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9H25
• Specificity: Recognizes human ADAM8. Reacts with aa498-653 of human ADAM8 which corresponds to the cysteine-rich and EGF-like domains. Shows no crossreactivity with recombinant human ADAM9, ADAM10, ADAM15, BACE or TACE.
• Purity/Purification: Purified by Protein G affinity chromatography. ; Endotoxin: <0.1EU/ug (LAL)
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
• Concentration: ~0.5mg/ml (after reconstitution) (varies by lot)

• Applicable Applications for anti-ADAM8 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Other applications not tested.
• Application Notes: Suitable for use in Flow Cytometry, Direct ELISA, Western Blot, and Immunoprecipitation.; Dilution: Flow Cytometry: 25ug/ml. 10ul of the diluted solution labels 1-2.5x10e5 cells in a total reaction volume <200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome.; Direct ELISA: 1ug/ml detects human ADAM8 (ecto) coated on 96 well plates. The detection limit is ~1ng/well.; Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~20ng/lane under non-reducing conditions.; Immunoprecipitation: 25ug/ml immunoprecipitates ADAM8 from conditioned cell culture media. The immunoprecipitated ADAM8 can be detected in western blots using an affinity-purified goat polyclonal antibody .
• Immunogen: Recombinant corresponding to aa158-653 from human ADAM8 Ectodomonain expressed in NSO cells.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ADAM8 antibody

Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3. ADAM8 is involved in protein processing at the cell surface and is also thought to act as a leukocyte differentiation marker.

Product Categories/Family for anti-ADAM8 antibody

Antibodies; Abs to Matrix Metalloproteinases

NCBI and Uniprot Product Information

• NCBI GI #: 109731932
• NCBI GeneID: 101
• UniProt Accession #: P78325; Q14C66
• Molecular Weight: 88,673 Da
• NCBI Official Full Name: ADAM8 protein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 8
• NCBI Official Symbol: ADAM8
• NCBI Official Synonym Symbols: MS2; CD156; MGC134985
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 8; cell surface antigen MS2; human leukocyte differentiation antigen; a disintegrin and metalloproteinase domain 8
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 8
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM8
• UniProt Synonym Gene Names: MS2
• UniProt Entry Name: ADAM8_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

ADAM8: Possible involvement in extravasation of leukocytes.

Protein type: Protease; Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: specific granule; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; podosome; dense core granule membrane

Molecular Function: protein binding; protein self-association; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; cell adhesion molecule binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell adhesion; cell morphogenesis; proteolysis; positive regulation of membrane protein ectodomain proteolysis; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; positive regulation of T cell differentiation in the thymus; positive regulation of protein kinase B signaling cascade; extracellular matrix disassembly; cell-cell adhesion; regulation of cell-cell adhesion; positive regulation of protein secretion; positive regulation of acute inflammatory response; positive regulation of bone resorption; negative regulation of neuron apoptosis; angiogenesis; inflammatory response; lymphocyte chemotaxis