ADAM8/CD156a Recombinant Protein | ADAM8 recombinant protein

ADAM8/CD156a Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA8120098

• Gene Names: ADAM8; MS2; CD156
• Purity: >95% as determined by SDS-PAGE
• Synonyms: ADAM8/CD156a; ADAM8/CD156a Protein; Human; Recombinant (ECD; His Tag); Human ADAM8/CD156 Protein (His Tag); CD156 Protein; CD156a Protein; MS2 Protein; ADAM metallopeptidase domain 8; ADAM8 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 12.5mM Tris, 5mM CaCl2, 75mM NaCl, 50% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser653

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Ile 17
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human ADAM8 (AAI15405.1)(Met1-Ser653) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ADAM8 recombinant protein

Background: ADAM8, also known as CD156, is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. ADAM8 is possiblely involved in extravasation of leukocytes As a metalloprotease, ADAM8 also may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma.

References

Yoshiyama K., et al.,(1997), CD156 (human ADAM8): expression, primary amino acid sequence, and gene location. Genomics 41:56-62.Ota T., et al., (2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.Deloukas P., et al.,(2004), The DNA sequence and comparative analysis of human chromosome 10.Nature 429:375-381.

NCBI and Uniprot Product Information

• NCBI GI #: 109731932
• NCBI GeneID: 101
• UniProt Accession #: P78325; B4DVM6; H0YL36; H0YLR0; H0YN39
• Molecular Weight: 88,771 Da
• NCBI Official Full Name: ADAM8 protein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 8
• NCBI Official Symbol: ADAM8
• NCBI Official Synonym Symbols: MS2; CD156
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 8; cell surface antigen MS2; human leukocyte differentiation antigen; a disintegrin and metalloproteinase domain 8
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 8
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM8
• UniProt Synonym Gene Names: MS2; ADAM 8
• UniProt Entry Name: ADAM8_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]

Uniprot Description

ADAM8: Possible involvement in extravasation of leukocytes.

Protein type: Protease; Membrane protein, integral; EC 3.4.24.-; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: specific granule; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; podosome; dense core granule membrane

Molecular Function: protein binding; protein self-association; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; cell adhesion molecule binding; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell adhesion; cell morphogenesis; proteolysis; positive regulation of membrane protein ectodomain proteolysis; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; positive regulation of T cell differentiation in the thymus; extracellular matrix disassembly; positive regulation of protein kinase B signaling cascade; cell-cell adhesion; regulation of cell-cell adhesion; positive regulation of protein secretion; positive regulation of acute inflammatory response; positive regulation of bone resorption; angiogenesis; negative regulation of neuron apoptosis; inflammatory response; lymphocyte chemotaxis