Rabbit anti-Human ADAM8/CD156a Polyclonal Antibody | anti-ADAM8 antibody

Anti-ADAM8/CD156a Antibody, Rabbit Polyclonal

Cat. Num. AAA8100423

• Gene Names: ADAM8; MS2; CD156
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: ADAM8/CD156a; Polyclonal Antibody; Anti-ADAM8/CD156a Antibody; Rabbit Polyclonal; ADAM8/CD156 Antibody; Rabbit PAb; Antigen Affinity Purified; ADAM metallopeptidase domain 8; Anti-CD156 Antibody; Anti-CD156a Antibody; Anti-MS2 Antibody; anti-ADAM8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ADAM8/CD156
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ADAM8 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:100-1:500; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human ADAM8/CD156
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human ADAM8/CD156, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human ADAM8 in human kidney with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of ADAM8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ADAM8 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Related Product Information for anti-ADAM8 antibody

ADAM8, also known as CD156, is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. ADAM8 is possiblely involved in extravasation of leukocytes As a metalloprotease, ADAM8 also may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma.

References

Yoshiyama K., et al., (1997), CD156 (human ADAM8): expression, primary amino acid sequence, and gene location. Genomics 41:56-62.Ota T., et al., (2004), Complete sequencing and characterization of 21, 243 full-length human cDNAs.Nat. Genet. 36:40-45.Deloukas P., et al., (2004), The DNA sequence and comparative analysis of human chromosome 10.Nature 429:375-381.

NCBI and Uniprot Product Information

• NCBI GI #: 109731932
• NCBI GeneID: 101
• UniProt Accession #: P78325; B4DVM6; H0YL36; H0YLR0; H0YN39
• Molecular Weight: 88,771 Da
• NCBI Official Full Name: ADAM8 protein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 8
• NCBI Official Symbol: ADAM8
• NCBI Official Synonym Symbols: MS2; CD156
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 8; cell surface antigen MS2; human leukocyte differentiation antigen; a disintegrin and metalloproteinase domain 8
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 8
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM8
• UniProt Synonym Gene Names: MS2; ADAM 8
• UniProt Entry Name: ADAM8_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]

Uniprot Description

ADAM8: Possible involvement in extravasation of leukocytes.

Protein type: Protease; Membrane protein, integral; EC 3.4.24.-; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: specific granule; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; podosome; dense core granule membrane

Molecular Function: protein binding; protein self-association; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; cell adhesion molecule binding; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell adhesion; cell morphogenesis; proteolysis; positive regulation of membrane protein ectodomain proteolysis; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; positive regulation of T cell differentiation in the thymus; extracellular matrix disassembly; positive regulation of protein kinase B signaling cascade; cell-cell adhesion; regulation of cell-cell adhesion; positive regulation of protein secretion; positive regulation of acute inflammatory response; positive regulation of bone resorption; angiogenesis; negative regulation of neuron apoptosis; inflammatory response; lymphocyte chemotaxis