Western Blot (WB)
(Western Blot analysis: The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with MBS607685 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human Wnt3a Monoclonal Antibody | anti-WNT3A antibody
Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)
Purified by Protein G chromatography.
Purified by Protein G chromatography.
Dilution: Western Blot: 1:1000-1:2000
Immunohistochemistry: 1:50-1:100. HEIR with citrate buffer.
Western Blot (WB)
(Western Blot analysis: The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with MBS607685 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(Western Blot analysis: The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with MBS607685 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunohistochemistry (IHC)
(Paraffin embedded sections of human breast cancer tissue were incubated with MBS607685 (1:50) for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using DAB.)
Immunohistochemistry (IHC)
(Paraffin embedded sections of human breast cancer tissue were incubated with MBS607685 (1:50) for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using DAB.)
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq]
Uniprot Description
WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 1q42
Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; early endosome membrane; endoplasmic reticulum lumen; Golgi lumen; extracellular region; plasma membrane
Molecular Function: protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; frizzled-2 binding
Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell proliferation in forebrain; positive regulation of transcription, DNA-dependent; positive regulation of receptor internalization; positive regulation of caspase activity; positive regulation of collateral sprouting in the absence of injury; Wnt receptor signaling pathway through beta-catenin; palate development; negative regulation of axon extension involved in axon guidance; Wnt receptor signaling pathway in forebrain neuroblast division; negative regulation of neurogenesis; neuron differentiation; mammary gland development; positive regulation of cell proliferation; positive regulation of B cell proliferation; hemopoiesis; heart looping; dorsoventral neural tube patterning; inner ear morphogenesis; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation
Research Articles on WNT3A
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Product Notes
The WNT3A wnt3a (Catalog #AAA607685) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Wnt3a can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:1000-1:2000 Immunohistochemistry: 1:50-1:100. HEIR with citrate buffer. Researchers should empirically determine the suitability of the WNT3A wnt3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wnt3a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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